Gene analysis of multiple oral bacteria by the polymerase chain reaction coupled with capillary polymer electrophoresis
Gene analysis of multiple oral bacteria by the polymerase chain reaction coupled with capillary polymer electrophoresis
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DOI：
10.1002/jssc.201501087
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发表时间：
2016-03-01
影响因子：
3.1
通讯作者：
Dou, Xiaoming
中科院分区：
文献类型：
--
作者：
Liu, Chenchen;Yamaguchi, Yoshinori;Dou, Xiaoming
毛细管聚合物电泳是一种很有前途的技术，可用于细菌、病毒和细胞样本的DNA分析。在本文中，我们提出了一种创新的毛细管聚合物电泳方案，用于定量聚合酶链反应产物。对内标法进行了改进，并应用于毛细管聚合物电泳。通过测量中间毛细管聚合物电泳实验的相对标准偏差，对改进的内标方案的精密度进行了评价。结果表明，相对标准偏差由12.4-15.1降低到0.6-2.3%。还实施了线性回归测试来验证我们的方案。改进的内标法具有良好的线性和鲁棒性。最后，通过对临床口腔样品进行一次毛细管电泳实验，验证了该方法的可行性。
Capillary polymer electrophoresis is identified as a promising technology for the analysis of DNA from bacteria, virus and cell samples. In this paper, we propose an innovative capillary polymer electrophoresis protocol for the quantification of polymerase chain reaction products. The internal standard method was modified and applied to capillary polymer electrophoresis. The precision of our modified internal standard protocol was evaluated by measuring the relative standard deviation of intermediate capillary polymer electrophoresis experiments. Results showed that the relative standard deviation was reduced from 12.4-15.1 to 0.6-2.3%. Linear regression tests were also implemented to validate our protocol. The modified internal standard method showed good linearity and robust properties. Finally, the ease of our method was illustrated by analyzing a real clinical oral sample using a one-run capillary polymer electrophoresis experiment.