3D rotational motion of an endocytic vesicle on a complex microtubule network in a living cell
3D rotational motion of an endocytic vesicle on a complex microtubule network in a living cell
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DOI：
10.1101/576702
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发表时间：
2019-03
期刊：
影响因子：
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通讯作者：
Seohyun Lee;Hideo Higuchi
中科院分区：
文献类型：
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作者：
Seohyun Lee;Hideo Higuchi
内吞囊泡在活细胞中的转运动力学包含着重要的生物医学信息。虽然已经揭示了马达蛋白的囊泡运动机制，但由于细胞骨架的复杂3D网络，理解活细胞中囊泡在细胞骨架上的精确运动一直被认为是具有挑战性的。在这里，我们指定的形状的三维相互作用之间的囊泡和微管，根据理论上估计的位置的微管和囊泡的轨迹数据在高的空间和时间精度。我们发现，在微管网络中，在类似的时间尺度上，囊泡显示出更频繁的方向变化，无论是在非常尖锐的角度还是钝角。有趣的是，当囊泡与相对较长（> 400 nm）的微管细丝相互作用时，经常观察到沿微管轴沿着的旋转运动，这意味着避障运动。我们的研究结果有望深入了解细胞内分子和复杂细胞骨架网络之间的实际3D相互作用。
The transport dynamics of endocytic vesicles in a living cell contains essential biomedical information. Although the movement mechanism of a vesicle by motor proteins has been revealed, understanding the precise movement of vesicles on the cytoskeleton in a living cell has been considered challenging, due to the complex 3D network of cytoskeletons. Here, we specify the shape of the 3D interaction between the vesicle and microtubule, based on the theoretically estimated location of the microtubule and the vesicle trajectory data acquired at high spatial and temporal precision. We detected that vesicles showed more frequent direction changes with either in very acute or in obtuse angles than right angles, on similar time scales in a microtubule network. Interestingly, when a vesicle interacted with a relatively longer (> 400 nm) microtubule filament, rotational movement along the axis of the microtubule was frequently observed, implying an obstacle-avoiding motion. Our results are expected to give in-depth insight into understanding the actual 3D interactions between the intracellular molecule and complex cytoskeletal network.