An important role for cytosol in the microsomal metabolism of N-nitrosodimethylamine to a mutagen: evidence for two different mutagenic metabolites.
An important role for cytosol in the microsomal metabolism of N-nitrosodimethylamine to a mutagen: evidence for two different mutagenic metabolites.
复制标题
细胞质在 N-亚硝基二甲胺向诱变剂的微粒体代谢中的重要作用：两种不同诱变代谢物的证据。
DOI：
10.1016/0304-3835(89)90178-x
复制
发表时间：
1989
期刊：
影响因子：
9.7
通讯作者：
Guttenplan,JB
中科院分区：
文献类型：
--
作者：
Guttenplan,JB
Microsomal-mediated mutagenesis induced by N-nitrosodimethylamine (NDMA) in Salmonella TA100 at neutral pH was only slightly affected by cytosol and was similar in its threshold type dose-response curve to mutagenesis induced by direct-acting N-nitroso-N-methyl compounds. However, mutagenesis in strain TA104 was greatly enhanced by cytosol and this mutagenesis did not exhibit a threshold. In the presence of microsomes alone NDMA was more potent in TA100 than TA104, but in the presence of microsomes plus cytosol (S-9 fraction) this order was reversed at the doses tested. A possible explanation for these results is that NDMA is metabolized by microsomes to a mutagen (presumably methyldiazonium ion; MDI) that is more potent in TA100 than in TA104, but in the presence of S-9 fraction a fraction of the NDMA is metabolized by a pathway leading to a different mutagen with a different specificity. The ratio of metabolism via these pathways appears to be dependent on pH.
