Complex Activity and Short-term Memories in Reciprocally Connected Cerebral Organoids
Complex Activity and Short-term Memories in Reciprocally Connected Cerebral Organoids
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相互连接的大脑类器官中的复杂活动和短期记忆
DOI：
10.1101/2021.02.16.431387
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Ikeuchi Yoshiho
中科院分区：
文献类型：
--
作者：
Osaki Tatsuya;Ikeuchi Yoshiho
人类大脑中的宏观轴突连接将信息和神经元活动分布在整个大脑中。虽然脑类器官技术最近提供了新的途径来研究人脑功能，通过体外复制大脑的小神经元网络，远距离区域之间的宏观连接的功能还没有研究与类器官。在这里，我们描述了由一束轴突连接的人类大脑类器官的神经活动。连接的类器官产生比传统的大脑类器官更强烈和复杂的振荡活动。光遗传学操作显示，连接的类器官可以响应外部刺激，并在短期内保持神经元活动的频率升高，表明连接的类器官表现出神经元网络的可塑性。我们的研究结果强调了区域间连接的功能重要性，并表明连接的类器官可用作研究人类大脑中宏观电路的发育和功能的强大工具。可以让研究人员开发和测试化合物，以前所未有的方式调节体外神经元网络模型的活性。
Macroscopic axonal connections in the human brain distribute information and neuronal activity across the brain. Although brain organoid technologies have recently provided novel avenues to investigate human brain function by replicating small neuronal networks of the brainin vitro, functionality of the macroscopic connections between distant regions has not been investigated with organoids. Here, we describe the neural activity of human cerebral organoids reciprocally connected by a bundle of axons. The connected organoids produced significantly more intense and complex oscillatory activity than conventional cerebral organoids. Optogenetic manipulations revealed that the connected organoids could respond to external stimuli and maintain the elevated frequency of neuronal activity for a short term, indicating that the connected organoids exhibit plasticity as a neuronal network. Our findings highlight functional importance of inter-regional connections and suggest that connected organoids can be used as a powerful tool for investigating the development and functions of macroscopic circuits in the human brain. can allow researchers to develop and test compounds to modulate activity ofin vitroneuronal network models in unprecedented ways.
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