‘Vascular Kallikrein’: A Kallikrein-Like Enzyme Present in Vascular Tissue of the Rat
‘Vascular Kallikrein’: A Kallikrein-Like Enzyme Present in Vascular Tissue of the Rat
复制标题
“血管激肽释放酶”：大鼠血管组织中存在的激肽释放酶样酶
DOI：
10.1042/cs063249s
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发表时间：
1982
期刊：
影响因子：
6
通讯作者：
M. Lama
中科院分区：
文献类型：
--
作者：
H. Nolly;M. Lama
1. 本研究旨在检测血管组织中是否存在一种类激肽释放酶。采用分离的生理盐水灌注的大鼠肠系膜动脉，在肽酶抑制剂存在的条件下，将动脉匀浆与热处理的狗血浆和柠檬酸/磷酸盐缓冲液（pH 8.5）一起孵育60分钟。
2. 类激肽释放酶在pH 7.0 - 9.0范围内表现出最佳活性。它释放激肽的速率与激肽释放酶和胰蛋白酶释放激肽的速率相似。该释放不受胰蛋白酶抑制剂与酶一起孵育的影响，但被抑肽酶完全抑制。
3. 动脉匀浆释放的激肽在化学和药理学特性上类似于缓激肽，并且通过使用特异性抑制激肽的抗体，其生物学作用被完全消除。
4. 这些结果表明，动脉组织含有一种酶，其物理化学特性与腺体来源的激肽释放酶非常相似。
1. The present study was undertaken to examine whether there is a kallikrein-like enzyme in vascular tissue. Isolated saline-perfused rat mesenteric arteries were used and arterial homogenate was incubated with heat-treated dog plasma and citrate/phosphate buffer (pH 8.5) in the presence of peptidase inhibitors for 60 min. 2. The kallikrein-like enzyme showed optimum activity in the range pH 7.0-9.0. The rate at which it releases kinin is similar to the release of kinin by kallikrein and trypsin. The release is unaffected by incubation of the enzyme with trypsin-inhibitors, but completely inhibited by aprotinin. 3. The kinin released by arterial homogenate resembles bradykinin in its chemical and pharmacological properties, and by using antibodies that specifically inhibit kinins, the biological action was completely abolished. 4. These results indicate that arterial tissue contains an enzyme with physicochemical characteristics quite similar to those of kallikreins of glandular origin.