Germ cells of the mouse testis express P450 aromatase.
Germ cells of the mouse testis express P450 aromatase.
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DOI：
10.1210/endo.132.3.8440194
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发表时间：
1993-03
期刊：
影响因子：
4.8
通讯作者：
H. Nitta;D. Bunick;R. Hess;L. Janulis;S. Newton;C. Millette;Y. Osawa;Y. Shizuta;K. Toda;J. Bahr
中科院分区：
文献类型：
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作者：
H. Nitta;D. Bunick;R. Hess;L. Janulis;S. Newton;C. Millette;Y. Osawa;Y. Shizuta;K. Toda;J. Bahr
睾丸内雌激素的产生多年来一直是一个颇具争议的话题。多项研究表明，支持细胞和间质细胞在不同的发育阶段都会产生雌激素。因此，我们进行了实验，将芳香酶（一种将雄激素转化为雌激素的细胞色素 P450 酶）定位在睾丸内。首先，使用针对纯化的人胎盘细胞色素 P450arom 产生的抗血清，通过免疫细胞化学将 P450 芳香酶 (P450arom) 定位于成年小鼠睾丸的生殖细胞中。在生发上皮中，P450arom 主要位于圆形精子细胞的高尔基体区域、伸长精子细胞的整个细胞质以及晚期精子细胞的鞭毛。其次，P450arom 在生发上皮内的定位得到了分离生殖细胞的蛋白质印迹分析的支持。第三，使用小鼠P450arom cDNA探针的Northern印迹分析表明小鼠P450arom的mRNA存在于睾丸生殖细胞中。第四，通过 3H2O 水测定法测量生殖细胞中的 P450arom 活性。基于这些观察，我们得出结论，生殖细胞是成年小鼠睾丸中雌激素合成的场所。
Estrogen production within the testis has been a subject of considerable controversy for many years. Several studies have shown that both Sertoli and Leydig cells produce estrogen during different stages of development. Therefore, we have conducted experiments to localize aromatase, a cytochrome P450 enzyme that converts androgen to estrogen, within the testis. First, P450 aromatase (P450arom) was localized in germ cells of the adult mouse testis by immunocytochemistry, using an antiserum generated against purified human placental cytochrome P450arom. In the germinal epithelium, P450arom was located primarily in the Golgi region of round spermatids, throughout the cytoplasm of elongating spermatids, and along the flagella of late spermatids. Second, localization of P450arom within the germinal epithelium was supported by Western blot analysis of isolated germ cells. Third, Northern blot analysis using a mouse P450arom cDNA probe indicated that the mRNA for the mouse P450arom was present in testicular germ cells. Fourth, P450arom activity was measured in germ cells by the 3H2O water assay. Based upon these observations, we conclude that germ cells are a site of estrogen synthesis in the adult mouse testis.