RNAi: Double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals
RNAi: Double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals
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DOI：
10.1016/s0092-8674(00)80620-0
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发表时间：
2000-03-31
期刊：
影响因子：
64.5
通讯作者：
Bartel, DP
中科院分区：
文献类型：
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作者：
Zamore, PD;Tuschl, T;Bartel, DP
双链RNA（dsRNA）通过称为RNA干扰（RNAi）的过程指导mRNA的序列特异性降解。利用最近开发的果蝇体外系统，我们研究了RNAi的分子机制。我们发现RNAi是ATP依赖性的，但与mRNA翻译解偶联。在RNAi反应期间，dsRNA的两条链被加工成长度为21-23个核苷酸的RNA区段。将dsRNA加工成小RNA片段不需要靶向mRNA。mRNA仅在与dsRNA相同的区域内被切割。切割发生在相隔21-23个核苷酸的位点，与dsRNA本身观察到的间隔相同，表明来自dsRNA的21-23个核苷酸片段指导mRNA切割。
Double-stranded RNA (dsRNA) directs the sequence-specific degradation of mRNA through a process known as RNA interference (RNAi). Using a recently developed Drosophila in vitro system, we examined the molecular mechanism underlying RNAi. We find that RNAi is ATP dependent yet uncoupled from mRNA translation. During the RNAi reaction, both strands of the dsRNA are processed to RNA segments 21-23 nucleotides in length. Processing of the dsRNA to the small RNA fragments does not require the targeted mRNA. The mRNA is cleaved only within the region of identity with the dsRNA. Cleavage occurs at sites 21-23 nucleotides apart, the same interval observed for the dsRNA itself, suggesting that the 21-23 nucleotide fragments from the dsRNA are guiding mRNA cleavage.