Hyperthermia enhancement of radiation response and inhibition of recovery from radiation damage in human glioma cells.
Hyperthermia enhancement of radiation response and inhibition of recovery from radiation damage in human glioma cells.
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热疗增强辐射反应并抑制人胶质瘤细胞辐射损伤的恢复。
DOI：
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发表时间：
1991
影响因子：
3.1
通讯作者：
L. Gerig
中科院分区：
文献类型：
--
作者：
G. Raaphorst;M. Feeley;C. Danjoux;V. DaSilva;L. Gerig
三个人胶质瘤细胞系的有效性进行了测试的热疗和热放射增敏。从增加辐射敏感性以及抑制辐射损伤恢复的角度评价了热敏化。测试的三种胶质瘤细胞系在辐射存活曲线上显示出大的肩部，并且具有恢复潜在致命辐射损伤的大能力。热疗引起所有三种细胞系的放射增敏，其主要特征是存活曲线肩的减少，存活曲线斜率中度降低。热疗的增敏作用与热疗的时间和温度有关。在45 ℃下持续60分钟，辐射存活曲线的肩部可以被完全消除，并且在各种条件下，在2戈伊水平下增强的细胞杀伤程度在10至20倍的范围内。当给予经辐照然后立即铺板或在铺板前延迟8小时以允许恢复的细胞高温时，发现高温在两种条件下均引起放射增敏。此外，当热疗剂量增加时，立即接种和延迟接种存活曲线之间的差异减小，并且在45 ℃下持续60分钟，这种差异完全消除，同时消除了存活曲线肩。这些数据表明，热疗可能在治疗人脑胶质瘤的放射增敏中发挥作用。
Three human glioma cell lines were tested for the effectiveness of hyperthermia and thermal radiosensitization. Thermal sensitization was evaluated from the perspective of increased radiosensitivity as well as inhibition of recovery from radiation damage. The three glioma cell lines tested showed large shoulders on the radiation survival curve and a large capacity for recovery of potentially lethal radiation damage. Hyperthermia caused radiosensitization in all three cell lines, which was primarily characterized by the reduction of the survival curve shoulder with moderate decreases in the survival curve slope. The radiosensitization was dependent on the time and temperature of the hyperthermia treatment. At 45 degree C for 60 min the shoulder of the radiation survival curve could be completely eliminated and the degree of enhanced cell killing at the 2 Gy level ranged from factors of 10 to 20 under the various conditions. When hyperthermia was given to cells which were irradiated and then plated immediately, or delayed for 8 h before plating to allow recovery, hyperthermia was found to cause radiosensitization under both conditions. In addition, when the hyperthermia dose was increased the difference between the immediate plating and the delayed plating survival curve decreased and for 45 degrees C for 60 min this difference was completely eliminated, concomitantly with the elimination of the survival curve shoulder. These data indicate that hyperthermia may play a role in radiosensitization for the treatment of human glioma.
放线菌酮对中国仓鼠卵巢细胞热诱导细胞杀伤、放射增敏和细胞 DNA 聚合酶活性丧失的影响。
影响因子：
3.4
作者：
Chu,GL;Dewey,WC
通讯作者：
Dewey,WC
高温对 CHO DNA 聚合酶 α 和 β 的影响。
影响因子：
3.4
作者：
Spiro,IJ;Denman,DL;Dewey,WC
通讯作者：
Dewey,WC