Proportions of acetyl-histone-positive hepatocytes indicate the functional status and prognosis of cirrhotic patients
Proportions of acetyl-histone-positive hepatocytes indicate the functional status and prognosis of cirrhotic patients
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DOI：
10.3748/wjg.v21.i21.6665
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发表时间：
2015-06-07
影响因子：
4.3
通讯作者：
Bu, Hong
中科院分区：
文献类型：
--
作者：
Zhou, Ping;Xia, Jie;Bu, Hong
目的：探讨乙酰组蛋白阳性肝细胞的比例是否可以作为肝功能恶化的标志物。方法：对过去15年诊断的3701例肝硬化患者中的611例进行筛查，并筛选出152例随访病例。制备石蜡组织微阵列用于免疫组织化学检查乙酰组蛋白表达。记录阳性肝细胞比例，并统计其与临床及实验室指标的相关性。结果：H2AK5ac(+)、H3K9/K14ac(+)、H3K27ac(+)肝细胞比例随着肝功能的恶化和血清肝损伤标志物水平的升高而逐渐升高。在随访病例中，H2AK5ac(+)、H3K9/K14ac(+)或H3K27ac(+)肝细胞> 70%的患者存活率具有统计学意义较低(P < 0.05)。此外，> 70%的H2AK5ac(+)或H3K27ac(+)肝细胞是总生存的强独立预测因子(P < 0.05)。结论：乙酰组蛋白阳性肝细胞的比例与肝硬化患者的肝功能和预后密切相关。
AIM: To investigate whether the proportions of acetylhistone- positive hepatocytes could be used as markers of deteriorating liver function.METHODS: In total, 611 cirrhotic cases from 3701 patients who were diagnosed during the past 15 years were screened, and 152 follow-up cases were selected. Paraffin tissue microarray was prepared for immunohistochemistry to examine acetyl-histone expression. The proportions of positive hepatocytes were recorded, and their correlations to clinical and laboratory indicators were analyzed statistically.RESULTS: The proportions of H2AK5ac(+), H3K9/K14ac(+) and H3K27ac(+) hepatocytes gradually increased with deteriorating liver function and with increasing levels of serum markers of liver injury. In the follow-up cases, patients with > 70% H2AK5ac(+), H3K9/K14ac(+) or H3K27ac(+) hepatocytes had statistically lower survival rates (P < 0.05). Furthermore, > 70% H2AK5ac(+) or H3K27ac(+) hepatocytes were strong independent predictors of overall survival (P < 0.05).CONCLUSION: The proportions of acetyl-histonepositive hepatocytes are closely associated with the liver function and prognosis of cirrhotic patients.