Diffusional mobility of Golgi proteins in membranes of living cells
Diffusional mobility of Golgi proteins in membranes of living cells
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DOI：
10.1126/science.273.5276.797
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发表时间：
1996-08-09
期刊：
影响因子：
56.9
通讯作者：
LippincottSchwartz, J
中科院分区：
文献类型：
--
作者：
Cole, NB;Smith, CL;LippincottSchwartz, J
高尔基膜蛋白在蛋白质和脂质的连续流动中保留在高尔基复合体中的机制还不清楚。用荧光光漂白恢复技术测定了与维多利亚水母（Aequorea victoria）绿色荧光蛋白融合的哺乳动物高尔基体膜蛋白在活HeLa细胞中的扩散迁移率。扩散系数范围从3 × 10（-9）平方厘米每秒到5 × 10（-9）平方厘米每秒，其中超过90%的嵌合蛋白是移动的。广泛的横向扩散的嵌合蛋白质之间发生高尔基堆。因此，嵌合体在高尔基体膜中快速且自由地扩散，这表明高尔基体靶向和保留这些分子不依赖于蛋白质固定。
The mechanism by which Golgi membrane proteins are retained with in the Golgi complex in the midst of a continuous flow of protein and lipid is not yet understood. The diffusional mobilities of mammalian Golgi membrane proteins fused with green fluorescent protein from Aequorea victoria were measured in living HeLa cells with the fluorescence photobleaching recovery technique. The diffusion coefficients ranged from 3 x 10(-9) square centimeters per second to 5 x 10(-9) square centimeters per second, with greater than 90 percent of the chimeric proteins mobile. Extensive lateral diffusion of the chimeric proteins occurred between Golgi stacks. Thus, the chimeras diffuse rapidly and freely in Golgi membranes, which suggests that Golgi targeting and retention of these molecules does not depend on protein immobilization.