Pyrethroid tolerance in Culex pipiens pipiens var molestus from Marin County, California
Pyrethroid tolerance in Culex pipiens pipiens var molestus from Marin County, California
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DOI：
10.1002/ps.799
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发表时间：
2004-04-01
影响因子：
4.1
通讯作者：
Cornel, AJ
中科院分区：
文献类型：
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作者：
McAbee, RD;Kang, KD;Cornel, AJ
2001年5月，从加州马林县采集的尖音库蚊品种骚扰者Forskal的幼虫样本，在瓶生物测定中发现，相对于标准敏感的致倦库蚊菌落（CQ 1），其对苄菊酯和氯菊酯的敲低反应降低。幼虫敏感性试验，使用CQ 1作为标准敏感性，表明马林蚊的LC 50抗性比为18.3，氯菊酯，溴氰菊酯和除虫菊酯的12和3.3。马林的殖民地建立，并迅速发展较高水平的电阻在几代后，暴露于氯菊酯作为幼虫。这些被选中的幼虫显示出交叉抗性，以高效氯氟氰菊酯以及滴滴涕。然而，在氯菊酯、苄菊酯和除虫菊酯存在下，成虫击倒时间。幼虫对拟除虫菊酯的耐受性增加后，部分和几乎完全逆转的敏感性，幼虫实现与S，S，S-三丁基三硫代磷酸和胡椒基丁醚（PBO），分别表明存在羧酸酯酶和P450单加氧酶介导的阻力。不敏感的靶点抗性（kdr）也检测到一些马林蚊通过使用现有的PCR为基础的诊断方法设计的Cxp pipiens L蚊子。羧酸酯酶介导的电阻支持使用新合成的新型拟除虫菊酯选择性底物的活性测定。瓶生物测定低估了水平的耐受性拟除虫菊酯的马林蚊相比，在现场试验中使用注册的拟除虫菊酯成虫与PBO和不。这项研究代表了美国野生蚊子种群对拟除虫菊酯耐药性的首次报告。(C)2003化学工业协会。
In May 2001 a sample of Culex pipiens pipiens variety molestus Forskal from Marin County, California, collected as larvae and reared to adults, was found to show reduced resmethrin and permethrin knock-down responses in bottle bioassays relative to a standard susceptible Cx pipiens quinquefasciatus Say colony (CQ1). Larval susceptibility tests, using CQ1 as standard susceptible, indicated that the Marin mosquitoes had LC50 resistance ratios of 18.3 for permethrin, 12 for deltamethrin and 3.3 for pyrethrum. A colony of Marin was established and rapidly developed higher levels of resistance in a few generations after exposure to permethrin as larvae. These selected larvae were shown to cross-resist to lambda-cyhalothrin as well as to DDT. However, adult knock-down time in the presence of permethrin, resmethrin and pyrethrum. was not increased after increase in tolerance to pyrethroids as larvae. Partial and almost complete reversion to susceptibility as larvae was achieved with S, S, S-tributylphosphorotrithioate and piperonyl butoxide (PBO), respectively, suggesting the presence of carboxylesterase and P450 monooxygenase mediated resistance. Insensitive target site resistance (kdr) was also detected in some Marin mosquitoes by use of an existing PCR-based diagnostic assay designed for Cx p pipiens L mosquitoes. Carboxylesterase mediated resistance was supported by use of newly synthesized novel pyrethroid-selective substrates in activity assays. Bottle bioassays gave underestimates of the levels of tolerance to pyrethroids of Marin mosquitoes when compared with mortality rates in field trials using registered pyrethroid adulticides with and without PBO. This study represents the first report of resistance to pyrethroids in a feral population of a mosquito species in the USA. (C) 2003 Society of Chemical Industry.