Effects of Salicylic Acid and Methyl Jasmonate Treatments on Flavonoid and Carotenoid Accumulation in the Juice Sacs of Satsuma Mandarin In Vitro
Effects of Salicylic Acid and Methyl Jasmonate Treatments on Flavonoid and Carotenoid Accumulation in the Juice Sacs of Satsuma Mandarin In Vitro
复制标题
DOI：
10.3390/app10248916
复制
发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
R. Yamamoto;G. Ma;Lancui Zhang;M. Hirai;M. Yahata;K. Yamawaki;T. Shimada;H. Fujii;T. Endo;M. Kato
中科院分区：
文献类型：
--
作者：
R. Yamamoto;G. Ma;Lancui Zhang;M. Hirai;M. Yahata;K. Yamawaki;T. Shimada;H. Fujii;T. Endo;M. Kato
水杨酸和茉莉酸是两种重要的植物激素，它们能触发植物的防御反应，调节植物体内生物活性物质的积累。研究了水杨酸（SA）和茉莉酸甲酯（MeJA）对温州蜜柑离体汁囊类黄酮和类胡萝卜素积累的影响。结果表明，SA处理能显著提高汁囊中圣草苷、罂粟苷、胭脂红苷和β-隐黄质的含量（p < 0.05）。相反，MeJA处理抑制了汁囊中类黄酮和类胡萝卜素的积累（p < 0.05）。基因表达结果表明，SA和MeJA处理中类黄酮和类胡萝卜素含量的变化在转录水平上受到高度调控。此外，在微阵列分析中鉴定了转录因子CitWRKY 70，其被SA处理诱导，而被MeJA处理抑制。在SA和MeJA处理中，CitWRKY 70的表达变化与类黄酮和类胡萝卜素生物合成关键基因的表达变化一致。这些结果表明CitWRKY 70可能参与了SA和MeJA处理对柑橘汁囊类黄酮和类胡萝卜素积累的调控。
Salicylic acid and jasmonic acid are two important plant hormones that trigger the plant defense responses and regulate the accumulation of bioactive compounds in plants. In the present study, the effects of salicylic acid (SA) and methyl jasmonate (MeJA) on flavonoid and carotenoid accumulation were investigated in the juice sacs of Satsuma mandarin in vitro. The results showed that SA treatment was effective to enhance the contents of eriocitrin, narirutin, poncirin, and β-cryptoxanthin in the juice sacs (p < 0.05). In contrast, the MeJA treatment inhibited flavonoid and carotenoid accumulation in the juice sacs (p < 0.05). Gene expression results showed that the changes of flavonoid and carotenoid contents in the SA and MeJA treatments were highly regulated at the transcriptional level. In addition, a transcriptional factor CitWRKY70 was identified in the microarray analysis, which was induced by the SA treatment, while suppressed by the MeJA treatment. In the SA and MeJA treatments, the change in the expression of CitWRKY70 was consistent with that of flavonoid and carotenoid biosynthetic key genes. These results indicated that CitWRKY70 might be involved in the regulation of flavonoid and carotenoid accumulation in response to SA and MeJA treatments in the juice sacs of citrus fruit.