Effects of vitamin C deficiency on the skin of the senescence marker protein-30 (SMP30) knockout mouse
Effects of vitamin C deficiency on the skin of the senescence marker protein-30 (SMP30) knockout mouse
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DOI：
10.1016/j.bbrc.2009.05.104
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发表时间：
2009-07-31
影响因子：
3.1
通讯作者：
Nishiyama, Toshio
中科院分区：
文献类型：
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作者：
Arai, Koji Y.;Sato, Yasunori;Nishiyama, Toshio
衰老标记蛋白-30（SMP 30）是维生素C（VC）合成所需的一种维生素内酯酶。我们使用SMP 30敲除（KO）小鼠研究了VC缺乏对小鼠皮肤的影响。将SMP 30 KO或野生型雄性小鼠在30日龄左右断奶，并喂食VC缺乏的饮食。他们被给予VC水或对照水。维生素C缺乏36天不影响皮肤羟脯氨酸含量，而维生素C缺乏60天则使皮肤羟脯氨酸含量下降。一些胶原mRNA的水平在各组之间存在差异，但与皮肤VC水平无关。在断奶后60天，VC缺陷型SMP 30 KO小鼠的表皮出现形态学异常。有趣的是，各组之间的毛发周期并不同步。这些数据表明小鼠皮肤对VC缺乏的敏感性低，并且VC参与了体内角质形成细胞功能和毛发周期的调节。(C)2009 Elsevier Inc. All rights reserved.
Senescence marker protein-30 (SMP30) is a gluconolactonase required for vitamin C (VC) synthesis. We examined effects of VC deficiency on the mouse skin using SMP30 knockout (KO) mice. SMP30 KO or wild type male mice were weaned around day 30 of age, and fed VC-deficient diet. They were given either VC water or control water. VC deficiency for 36 days did not affect skin hydroxyproline contents, while VC deficiency for 60 days decreased the hydroxyproline levels. Levels of some collagen mRNAs were different among the groups, but did not correlate with skin VC levels. The epidermis was morphologically abnormal in VC-deficient SMP30 KO mouse at 60 days after the weaning. Interestingly, the hair cycle was not synchronized among the groups. These data suggest low susceptibility of the mouse skin to VC deficiency and involvement of VC in the regulation of keratinocyte function and hair cycle in vivo. (C) 2009 Elsevier Inc. All rights reserved.