Individualization of pubic hair bacterial communities and the effects of storage time and temperature
Individualization of pubic hair bacterial communities and the effects of storage time and temperature
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DOI：
10.1016/j.fsigen.2016.09.006
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发表时间：
2017-01-01
影响因子：
3.1
通讯作者：
Gibson, Greg
中科院分区：
文献类型：
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作者：
Williams, Diana W.;Gibson, Greg
微生物遗传学在法医学中的一个潜在应用是使用高通量测序检测性交期间个体之间的阴毛微生物组的转移。除了显示阴毛微生物组是否个性化的主要需求外，在刑事案件中使用微生物组之前必须解决的一个方面涉及储存对法医检测回收样品微生物组的影响。为了测试短期储存的效果，从志愿者收集阴毛样品，并与基线样品一起在室温（类似于20摄氏度）、冷藏（4摄氏度）和冷冻（-20摄氏度）下储存1周、2周、4周和6周沿着。个体微生物特征（R-2 = 0.69）和性别（R-2 = 0.17）是样本间变异的最大来源。由于这种变化，在这个样本集中，可以使用随机森林监督分类来预测个体和性别，总体错误率分别为2.7% +/- 5.8%和1.7% +/-5.2%。没有统计学上的显着差异归因于采样时间或个人内的存储温度。对更大样本集的进一步研究将量化个体特征的时间一致性，并确定检测性伴侣之间的转移是否合理。对于短期存储（
A potential application of microbial genetics in forensic science is detection of transfer of the pubic hair microbiome between individuals during sexual intercourse using high-throughput sequencing. In addition to the primary need to show whether the pubic hair microbiome is individualizing, one aspect that must be addressed before using the microbiome in criminal casework involves the impact of storage on the microbiome of samples recovered for forensic testing. To test the effects of short-term storage, pubic hair samples were collected from volunteers and stored at room temperature (similar to 20 degrees C), refrigerated (4 degrees C), and frozen (-20 degrees C) for 1 week, 2 weeks, 4 weeks, and 6 weeks along with a baseline sample. Individual microbial profiles (R-2 = 0.69) and gender (R-2 = 0.17) were the greatest sources of variation between samples. Because of this variation, individual and gender could be predicted using Random Forests supervised classification in this sample set with an overall error rate of 2.7% +/- 5.8% and 1.7% +/- 5.2%, respectively. There was no statistically significant difference attributable to time of sampling or temperature of storage within individuals. Further work on larger sample sets will quantify the temporal consistency of individual profiles and define whether it is plausible to detect transfer between sexual partners. For short-term storage (