Ethidium bromide: A general purpose fluorescent stain for nucleic acid in bacteria and eucaryotes and its use in microbial ecology studies
Ethidium bromide: A general purpose fluorescent stain for nucleic acid in bacteria and eucaryotes and its use in microbial ecology studies
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溴化乙锭：细菌和真核生物核酸的通用荧光染色剂及其在微生物生态学研究中的应用
DOI：
10.1016/0038-0717(80)90005-x
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发表时间：
1980
影响因子：
9.7
通讯作者：
D. Roser
中科院分区：
文献类型：
--
作者：
D. Roser
荧光染料溴化乙锭（EB）与双链DNA结合，在紫外线辐射激发下发出荧光。当应用在水溶液浓度范围从2毫克l-1至1克l-1 EB强烈染色所有真核和原核细胞类型的测试。它优先集中在含有DNA的原核细胞和真核细胞的细胞核和其他细胞质成分中。它不会像含有核酸的材料那样强烈地染色细胞壁或惰性材料，例如粘土和沙子。EB作为染色剂的潜在应用包括检测植物根核以及检查土壤和泥浆制剂中的细菌和真菌。
The fluorochrome ethidium bromide (EB) combines with double-stranded DNA and fluoresces when excited by u.v. radiation. When applied at aqueous concentrations ranging from 2 mg l-1 to 1 g l-1 EB strongly stained all eucaryotic and procaryotic cell types tested. It concentrated preferentially in procaryotic cells containing DNA and in the nucleus and other cytoplasmic components of eucaryotic cells. It did not stain cell walls or inert materials, e.g. clay and sand, as intensely as material containing nucleic acid. Potential applications of EB as a stain include the detection of plant root nuclei and the examination of bacteria and fungi in soil and mud preparations.