Comparative Evaluation of Different Sanitizers Against Listeria monocytogenes Biofilms on Major Food-Contact Surfaces
Comparative Evaluation of Different Sanitizers Against Listeria monocytogenes Biofilms on Major Food-Contact Surfaces
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DOI：
10.3389/fmicb.2019.02462
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发表时间：
2019-11-07
影响因子：
5.2
通讯作者：
Zhu, Mei-Jun
中科院分区：
文献类型：
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作者：
Hua, Zi;Korany, Ahmed Mahmoud;Zhu, Mei-Jun
食品接触面污染被认为是近期L。在焦糖苹果和哈密瓜中爆发单核细胞增多症，突出了清洁和消毒食品接触表面的重要性，以确保新鲜农产品的微生物安全。本研究评价了四种常用化学消毒剂在实际浓度下对乳酸杆菌的消毒效果。主要食品接触表面上的单核细胞增生生物膜包括不锈钢、低密度聚乙烯（LDPE）、聚氯乙烯（PVC）、聚酯（PET）和橡胶。总体上，对L.通过增加季铵化合物（QAC）、氯和二氧化氯的浓度或将处理时间从1延长至5 min来增强单核细胞增多症生物膜。400 ppm QAC、5.0 ppm二氧化氯和200 ppm氯的5 min处理降低了3.0-3.7、2.4-2.7和2.6-3.8 log（10）CFU/片L。单核细胞增生生物膜依赖于表面。过氧乙酸（PAA）在160和200 ppm显示出类似的抗微生物效力对生物膜无论是在1分钟或5分钟的接触。200 ppm PAA处理5分钟导致L.单核细胞增多症生物膜的测试表面。表面材料对QAC和氯气的杀菌效果影响较大，对PAA和二氧化氯的影响较小，有机物污染则影响杀菌剂对乳酸杆菌的杀菌效果。单核细胞增生生物膜独立的食品接触表面。本研究的数据为食品加工/包装设施中食品接触表面的有效消毒提供了实用指导。
Contaminated food-contact surfaces are recognized as the primary reason for recent L. monocytogenes outbreaks in caramel apples and cantaloupes, highlighting the significance of cleaning and sanitizing food-contact surfaces to ensure microbial safety of fresh produce. This study evaluated efficacies of four commonly used chemical sanitizers at practical concentrations against L. monocytogenes biofilms on major food-contact surfaces including stainless steel, low-density polyethylene (LDPE), polyvinyl chloride (PVC), polyester (PET), and rubber. In general, efficacies against L. monocytogenes biofilms were enhanced by increasing concentrations of quaternary ammonium compound (QAC), chlorine, and chlorine dioxide, or extending treating time from 1 to 5 min. The 5-min treatments of 400 ppm QAC, 5.0 ppm chlorine dioxide, and 200 ppm chlorine reduced 3.0-3.7, 2.4-2.7, and 2.6-3.8 log(10) CFU/coupon L. monocytogenes biofilms depending on surfaces. Peroxyacetic acid (PAA) at 160 and 200 ppm showed similar antimicrobial efficacies against biofilms either at 1- or 5-min contact. The 5-min treatment of 200 ppm PAA caused 4.0-4.5 log(10) CFU/coupon reduction of L. monocytogenes biofilms on tested surfaces. Surface material had more impact on the efficacies of QAC and chlorine, less influence on those of PAA and chlorine dioxide, while organic matter soiling impaired sanitizer efficacies against L. monocytogenes biofilms independent of food-contact surfaces. Data from this study provide practical guidance for effective disinfection of food-contact surfaces in food processing/packing facilities.