Heterosigma akashiwo HADAのB群ビタミン要求に関する増殖生理
Heterosigma akashiwo HADAのB群ビタミン要求に関する増殖生理
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赤潮异形藻 HADA 的生长生理学 B 族维生素需求量
DOI：
10.2331/suisan.50.1505
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发表时间：
1984
影响因子：
0.4
通讯作者：
幸彦 畑
中科院分区：
文献类型：
--
作者：
敏隆 西島;幸彦 畑
对赤潮浮游植物 Heterosigma akashiwo HADA（针藻纲）生长的生理特性进行了检查，特别参考了无菌克隆培养中 B 族维生素的需求。该藻类广盐性，喜食浓度较高的营养物质。结果发现，只有维生素 B12 对生长至关重要，并且生长速率和最终细胞产量取决于培养基中维生素 B12 的浓度。维生素 B12 半饱和常数非常小：0.37-0.60ng/l。然而，它需要20ng/l的更高浓度才能维持最大生长产量。最大特定增长率为 0.62-0.82 day-1。维生素B12的最小细胞配额为8.9×10-5pg/细胞或24.2分子/μm3细胞体积。在富含维生素 B12 的培养基中生长的细胞能够积累高达最低配额的 50 倍。这些结果表明，海水中的维生素B12可能是控制赤潮红藻生长及其种群发展为赤潮的最主要因素之一。
Physiological characteristics on the growth of Heterosigma akashiwo HADA (Raphidophyceae), a red tide phytoplankton, were examined with special reference to B group vitamin requirements in the axenic clonal culture. The alga was wide-range euryhaline, and preferred relatively high concentration of nutrients. It was found that only vitamin B12 was essential for the growth, and that growth rates and final cell yields were dependent on the vitamin B12 concentrations in the media. Vitamin B12 halfsaturation constants were very small: 0.37-0.60ng/l. However, it needed the higher concentration of 20ng/l to maintain maximum growth yields. Maximum specific growth rates were 0.62-0.82 day-1. The minimum cell quota of vitamin B12 was 8.9×10-5pg/cell or 24.2 molecules/μm3 of the cell volume. Cells grown in the vitamin B12-rich media were able to accumulate it up to 50-fold as much as the minimum quota. These results suggest that vitamin B12 in seawater may be one of the most principal factors controlling the growth of H. akashiwo, and consequently the development of its population to a red tide.