Precision of scoring radiation-induced chromosomal aberrations and micronuclei by unexperienced scorers.
Precision of scoring radiation-induced chromosomal aberrations and micronuclei by unexperienced scorers.
复制标题
由经验不足的评分员对辐射引起的染色体畸变和微核进行评分的精度。
DOI：
10.1080/09553002.2019.1625462
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发表时间：
2019
影响因子：
2.6
通讯作者：
Galecki M
中科院分区：
文献类型：
--
作者：
Galecki M
目的：剂量评估在放射事故中起着重要的作用，可以通过对电离辐射引起的细胞染色体形态结构变化进行评分来进行。这种测试的结果会受到评分员经验的影响，因此，当需要快速分析大量数据时，建议使用简单易学的分析方法。本研究的目的是比较两个放射生物学检测-染色体畸变和微核-由没有经验的评分员与参考值产生的expert.Materials和方法：每个参与者的欧盟资助的为期两周的放射生物学课程被要求评分中国仓鼠卵巢细胞暴露于γ射线高达4戈伊。研究了学生和专家在每个剂量下得分的一致性以及学生之间剂量-反应曲线参数的一致性。结果：微核试验往往比染色体畸变评分更快、更容易学习。然而，这两个试验进行了无经验的学生表现出合理的剂量反应curves.Conclusions：在一个大的辐射事故，涉及许多伤亡的情况下，无经验的记分员将支持细胞遗传学生物剂量学的生物剂量分流的过程。
Purpose:Dose assessment plays an important role in case of radiological accidents and can be performed by scoring structural changes of chromosome morphology induced in cells by ionizing radiation. The results of such a test are biased by scorer experience, therefore, simple to learn assays are recommended to be used when fast analysis of a large amount of data is needed. The aim of this study was to compare the performance of two radiobiological assays – chromosomal aberrations and micronuclei – by unexperienced scorers with the reference values generated by an expert.Materials and methods:Each participant of an EU-funded two-week radiobiology course was asked to score Chinese hamster ovary cells exposed to gamma radiation up to 4 Gy. The congruence of students’ and expert’s scores at each dose and the coherence of the dose-response curve parameters between the students were investigated.Results:Micronucleus test tended to be faster and easier to learn than scoring chromosomal aberrations. However, both assays carried out by inexperienced students showed reasonable dose-response curves.Conclusions:In the case of a large radiological accident involving many casualties, the unexperienced scorers would support the process of biodosimetric triage by cytogenetic biological dosimetry.