A tRNA half modulates translation as stress response in Trypanosoma brucei
A tRNA half modulates translation as stress response in Trypanosoma brucei
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DOI：
10.1038/s41467-018-07949-6
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发表时间：
2019-01-10
影响因子：
16.6
通讯作者：
Polacek, Norbert
中科院分区：
文献类型：
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作者：
Fricker, Roger;Brogli, Rebecca;Polacek, Norbert
在缺乏广泛的转录控制机制的情况下，致病性寄生虫布鲁氏锥虫主要依赖翻译调控来协调基因表达。然而，对调节蛋白质生物合成的分子见解是稀疏的。本文分析了不同生长条件和不同生命阶段布鲁氏体核糖体的小非编码RNA （ncRNA）相互作用。核糖体相关的ncrna最近被认为是核糖体功能的前所未有的调节器。我们的数据表明，tRNA(Thr) 3'一半是在营养剥夺过程中产生的，并成为最丰富的tRNA衍生RNA片段（tdr）之一。tRNA（Thr）一半与核糖体和多体结合，在饥饿条件停止后的应激恢复过程中通过促进mRNA装载来刺激翻译。阻断或消耗内源性tRNA（Thr）一半可以减轻体内和体外的这种刺激作用。布鲁氏绦虫及其近亲缺乏哺乳动物中用于tRNA半加工的酶，因此暗示这些寄生虫具有独特的tdR生物发生机制。
In the absence of extensive transcription control mechanisms the pathogenic parasite Trypanosoma brucei crucially depends on translation regulation to orchestrate gene expression. However, molecular insight into regulating protein biosynthesis is sparse. Here we analyze the small non-coding RNA (ncRNA) interactome of ribosomes in T. brucei during different growth conditions and life stages. Ribosome-associated ncRNAs have recently been recognized as unprecedented regulators of ribosome functions. Our data show that the tRNA(Thr) 3'half is produced during nutrient deprivation and becomes one of the most abundant tRNA-derived RNA fragments (tdRs). tRNA(Thr) halves associate with ribosomes and polysomes and stimulate translation by facilitating mRNA loading during stress recovery once starvation conditions ceased. Blocking or depleting the endogenous tRNA(Thr) halves mitigates this stimulatory effect both in vivo and in vitro. T. brucei and its close relatives lack the well-described mammalian enzymes for tRNA half processing, thus hinting at a unique tdR biogenesis in these parasites.