Major histocompatibility complex (MHC) tetramer technology: an evaluation.
Major histocompatibility complex (MHC) tetramer technology: an evaluation.
复制标题
DOI：
10.1177/1099800402238332
复制
发表时间：
2002-10-01
影响因子：
2.5
通讯作者：
Strickland, Ora L
中科院分区：
文献类型：
--
作者：
Constantin, Carolyn M;Bonney, Elizabeth E;Strickland, Ora L
单细胞分析目前更倾向于量化T细胞的反应。用荧光标记的四聚体主要组织相容性复合体(MHC)/肽复合物对抗原特异性T细胞进行染色，极大地提高了在单细胞水平上评估免疫反应的细胞动力学的能力。本文综述了MHC四聚体技术，给出了MHC四聚体的定义，讨论了MHC四聚体是如何制造的，概述了该技术的优点，并将其与其他评估免疫反应的方法进行了比较和对比，并描述了目前的应用。
Single-cell assays are currently favored to quantitate T-cell responses. Staining antigen-specific T-cells with fluorescently labeled tetrameric major histocompatibility complex (MHC)/peptide complexes has greatly enhanced the ability to assess the cellular dynamics of an immune response at the single-cell level. This article reviews MHC tetramer technology, defining it, discussing how MHC tetramers are made, outlining the benefits of this technology, comparing and contrasting it to other methods for evaluating immune responses, and describing current applications.