Short- and long-term effects of copper on the rosy barb (Puntius conchonius Ham.).
Short- and long-term effects of copper on the rosy barb (Puntius conchonius Ham.).
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铜对玫瑰色倒刺（Puntius conchonius Ham.）的短期和长期影响。
DOI：
10.1016/0147-6513(92)90079-i
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发表时间：
1992
影响因子：
6.8
通讯作者：
J. Pande
中科院分区：
文献类型：
--
作者：
T. S. Gill;H. Tewari;J. Pande
将玫瑰色倒钩（Puntius conchonius）短期（48小时）和长期（8周）暴露于铜（Cu）中，并检查对血液和组织中酶活性和生化变量的影响。体内暴露于 571 μg CuSO 4/升（96 小时中位耐受限 (TLm)）48 小时，不同程度地刺激选定组织中的酸性磷酸酶 (AcP)、碱性磷酸酶 (AIP)（肝脏除外）和乙酰胆碱酯酶活性。体内谷丙转氨酶和乳酸脱氢酶(LDH)(心脏除外)活性均受到不同程度的抑制。在体外，10−6 M Cu 的存在抑制了所检查组织中的酶活性，但有一些例外，例如卵巢和肠道中的 AcP，肝脏、鳃、肠道和睾丸中的 AIP，以及肝脏中的 LDH。暴露于 190 μg CuSO 4/升（1 3 96 小时 TLm）的鱼出现高血糖、高乳酸血症、高蛋白血症、血液游离脂肪酸 (FFA) 水平升高和低胆固醇血症。对组织的影响包括糖原分解（肝脏和骨骼肌）、糖原生成（脑和心脏）、肝蛋白显着升高、肝脏和骨骼肌中 FFA 的积累以及肝脏和性腺胆固醇含量的降低。 8周后，生化变量（血液和肝蛋白水平除外）出现恢复趋势。
The rosy barb (Puntius conchonius) was exposed to copper (Cu) for short (48 hr) and long (8 weeks) terms and effects on enzyme activities and biochemical variables in the blood and tissues were examined. In vivo exposure to 571 μg CuSO 4/liter (96-hr median tolerance limit (TLm)) for 48 hr stimulated to varying degrees acid phosphatase (AcP), alkaline phosphatase (AIP)(except in the liver), and acetylcholinesterase activities in selected tissues. The alanine aminotransferase and lactic dehydrogenase (LDH)(except in the heart) activities were inhibited to varying degrees in vivo. In vitro, the presence of 10− 6 M Cu suppressed enzyme activities in the tissues examined, with a few exceptions such as AcP in ovaries and gut, AIP in liver, gills, gut, and testes, and LDH in liver. Hyperglycemia, hyperlactemia, hyperproteinemia, elevated blood free fatty acid (FFA) levels, and hypocholesterolemia were manifested in the fish exposed to 190 μg CuSO 4/liter (1 3 96-hr TLm). Effects on the tissues included glycogenolysis (liver and skeletal muscles), glycogenesis (brain and heart), a marked rise in hepatic proteins, accumulation of FFAs in liver and skeletal muscles, and reduction in hepatic and gonadal cholesterol contents. After 8 weeks, a trend toward recovery was noted in the biochemical variables (except blood and hepatic protein levels).