Stressor-dependent regulation of the heat shock response in zebrafish, Danio rerio
Stressor-dependent regulation of the heat shock response in zebrafish, Danio rerio
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DOI：
10.1016/s1095-6433(03)00033-3
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发表时间：
2003-04-01
影响因子：
2.3
通讯作者：
Nikinmaa, M
中科院分区：
文献类型：
--
作者：
Airaksinen, S;Råbergh, CMI;Nikinmaa, M
热休克转录因子（HSFs）调节热休克蛋白（Hsps）的表达。我们以前已经表明，在斑马鱼中的一个独特的亚型，zHSF 1b，消失伴随着热休克诱导的Hsp 70表达。为了表征zHSF 1a和zHSF 1b亚型在体内应激反应调节中的作用，我们进行了镉（10-100 μ M）和铜（10-30 μ M）暴露，以确定HSF 1b的消失是否是热应激特异性的。在4小时的金属暴露后，我们分析了热休克蛋白70，zHSF 1a，zHSF 1b和金属硫蛋白（MT）的表达在斑马鱼的肝脏，性腺和鳃的逆转录聚合酶链反应。虽然镉是一种已知的热休克蛋白诱导剂，它并没有影响热休克蛋白70的表达显着在所研究的组织。铜暴露后，在肝脏和性腺中观察到热休克蛋白70的诱导，但在鳃中没有。两种金属均不影响zHSF 1a/B比。镉和铜暴露引起的MT，调节金属稳态和解毒，确认组织进行金属负荷的上调。因此，热休克蛋白70似乎是更弱的金属暴露后诱导比热休克和HSF 1亚型可能参与应激特异性调节热休克蛋白70。(C)2003年爱思唯尔科学公司All rights reserved.
Heat shock transcription factors (HSFs) regulate expression of heat shock proteins (Hsps). We have previously shown that in zebrafish a unique isoform, zHSF1b, disappears concomitant with heat shock-induced Hsp70 expression. To characterize the role of zHSF1a and zHSF1b isoforms in the, regulation of the stress response in vivo, we have carried out cadmium (10-100 muM) and copper (10-30 muM) exposures in order to specify whether the disappearance of HSF1b is specific for heat stress. After 4-h metal exposures we analyzed the expression of hsp70, zHSF1a, zHSF1b and metallothionein (MT) by reverse transcriptase polymerase chain reaction in zebrafish liver, gonads and gills. Although cadmium is a known inducer of Hsps, it did not affect hsp70 expression significantly in the studied tissues. Induction of hsp70 was observed upon copper exposure in liver and gonads, but not in gills. Neither metal affected the zHSF1a/b ratio. Both cadmium and copper exposure caused upregulation of MT, regulator of metal homeostasis and detoxification, confirming that the tissues were subjected to metal loads. Thus, hsp70 appears to be more weakly induced upon metal exposure than in response to heat shock and HSF1 isoforms may participate in stressor-specific regulation of hsp70. (C) 2003 Elsevier Science Inc. All rights reserved.