Inability to detect fetal metaphases in flow-sorted lymphocyte cultures based on maternal-fetal HLA differences.
Inability to detect fetal metaphases in flow-sorted lymphocyte cultures based on maternal-fetal HLA differences.
复制标题
无法根据母胎 HLA 差异在流式分选淋巴细胞培养物中检测胎儿中期。
DOI：
10.1159/000263755
复制
发表时间：
1993
影响因子：
2.2
通讯作者：
Elias,S
中科院分区：
文献类型：
--
作者：
Tharapel,AT;Jaswaney,VL;Dockter,ME;Wachtel,SS;Chandler,RW;Simpson,JL;Shulman,LP;Meyers,CM;Elias,S
从母体血液中分离胎儿细胞可以为产前诊断提供一种不会危及胎儿的手段。在这项研究中，我们尝试对根据父母的人类白细胞抗原差异进行分类的候选胎儿细胞流进行细胞遗传学分析。中期显示46，XY或非整倍体，并与产前诊断(即羊膜穿刺术、绒毛取样)相一致，可能是胎儿起源。采集了78名孕妇及其配偶的血样。在18例恢复中期分裂相的人类白细胞抗原信息病例中，15例涉及46，XY或非整倍体胎儿。在这15个案例中，分析了2483个中期。所有的中期都是46，XX。因此，流动分选胎儿细胞的细胞遗传学分析可能需要强调的不是中期分析，而是染色体特异探针的原位杂交。
Separation of fetal cells from maternal blood could provide a means for prenatal diagnosis that would not endanger the fetus. In this pursuit, we attempted cytogenetic analysis of candidate fetal cells flow sorted on the basis of parental HLA disparity. Metaphases showing 46, XY or aneuploidy and concordant with prenatal diagnostic studies (i.e., amniocentesis, chorionic villus sampling) would presumably be fetal in origin. Blood samples were obtained from 78 pregnant women and their partners. Among 18 HLA informative cases in which metaphases were recovered, 15 involved fetuses that were 46, XY or aneuploid. From these 15 cases, 2,483 metaphases were analyzed. All metaphases were 46, XX. Cytogenetic analysis of flow-sorted fetal cells thus probably will need to emphasize not metaphase analysis but in situ hybridization with chromosome-specific probes.