Regulating Coupling Efficiency of REDV by Controlling Silk Fibroin Structure for Vascularization
Regulating Coupling Efficiency of REDV by Controlling Silk Fibroin Structure for Vascularization
复制标题
通过控制血管化丝素蛋白结构来调节 REDV 的偶联效率
DOI：
10.1021/acsbiomaterials.7b00553
复制
发表时间：
2017
影响因子：
5.8
通讯作者：
Yubo Fan
中科院分区：
文献类型：
--
作者：
Danyu Yao;Ge Peng;Zhiyong Qian;Yimeng Niu;Haifeng Liu;Yubo Fan
工程组织的受控和快速血管化仍然是组织工程的主要挑战之一。支架上肽和其他生物活性分子的固定已被证明能够改善血管化。然而，支架表面修饰肽的密度是影响血管化的重要因素。因此，调节肽的偶联效率可能是调节血管化的有效途径。在本研究中，制备了具有不同二级结构的二维（2D）丝素蛋白（SF）膜和三维（3D）多孔SF支架，并与Arg-Glu-Asp-Val（REDV）肽偶联。与高结晶支架相比，更多的肽以2D和3D形式结合在低结晶支架上，导致更多的内皮细胞粘附在低结晶SF支架上。此外，体内血管生成实验表明，低结晶支架在植入28天后显示出更高的血管密度，是高结晶组的1.4倍。结果表明，肽密度可以通过SF结构控制，并且用REDV肽修饰的低结晶SF支架可以成为组织工程应用中诱导血管生成的潜在候选者。
Controlled and rapid vascularization of engineered tissues remains one of the main challenges for tissue engineering. The immobilization of peptides and other bioactive molecules on the scaffolds has been demonstrated to be able to improve vascularization. However, the density of peptides modified on the scaffold surface is an important factor influencing vascularization. Thus, regulating the coupling efficiency of peptides may be an effective way to adjust vascularization. In this study, two-dimensional (2D) silk fibroin (SF) films and three-dimensional (3D) porous SF scaffolds with different secondary structure were prepared and coupled with Arg-Glu-Asp-Val (REDV) peptide. Compared with the high crystalline scaffolds, more peptides were bound on the scaffolds with low crystalline both in 2D and 3D forms with the result that more endothelial cells adhered on the low crystalline SF scaffolds. In addition, the in vivo angiogenic assays demonstrated that the low crystalline scaffolds showed higher blood vessel density after 28 days of implantation, which was 1.4-times as much as that of the high crystalline group. The results indicated that the peptide density could be controlled by SF structure and that the low crystalline SF scaffolds modified with REDV peptide could be a potential candidate for inducing angiogenesis in tissue engineering applications.