Novel host immune evasion strategy of the endoparasitoid Drino inconspicuoides
Novel host immune evasion strategy of the endoparasitoid Drino inconspicuoides
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内寄生蜂 Drino inconspicuoides 的新型宿主免疫逃避策略
DOI：
10.1017/s0007485318001049
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发表时间：
2019
影响因子：
1.9
通讯作者：
Furukawa S.
中科院分区：
文献类型：
--
作者：
Yamashita K.;Zhang K.;Ichiki R.T.;Nakamura S.;Furukawa S.
速蝇（双翅目：速蝇科）是一种卵生类内寄生昆虫，其幼虫在寄主血液中发育，避开寄主免疫系统。在本研究中，我们研究了该物种在侵染宿主Mythimna separata（鳞翅目：夜蛾科）时的免疫逃避机制。通过基因组聚合酶链式反应分析，我们发现了一种独特的“斗篷”，包裹着渗透到宿主体内的D. inoides幼虫，并确定这种结构来自宿主而不是速蛋白。这个“斗篷”包含了来自宿主的血细胞和脂肪体细胞，血细胞首先聚集在幼虫周围，然后脂肪体细胞覆盖血细胞层，随后两者混合。被包裹在“斗篷”中的活的不引人注目的幼虫没有被黑化，而被包裹的死幼虫则被黑化，这表明这种结构有助于避免宿主的免疫反应。
The tachinid fly Drino inconspicuoides (Diptera: Tachinidae) is an ovolarviparous endoparasitoid whose larvae develop in the host haemocoel and avoids the host immune system. In this study, we investigated the immune evasion mechanisms of this species during infestation in the host Mythimna separata (Lepidoptera: Noctuidae). We discovered a unique ‘cloak’ that surrounded D. inconspicuoides larvae that penetrated into the host and determined through genomic polymerase chain reaction analysis that this structure originated from the host rather than the tachinid. The ‘cloak’ contained both haemocytes and fat body cells from the host, with the haemocytes assembling around the larvae first and the fat body cells then covering the haemocyte layer, following which the two mixed. Living D. inconspicuoides larvae that were wrapped in the ‘cloak’ were not melanized whereas encapsulated dead larvae were melanized, suggesting that this structure contributes to the avoidance of host immune reactions.