MPB59, a widely cross-reacting protein of Mycobacterium bovis BCG.
MPB59, a widely cross-reacting protein of Mycobacterium bovis BCG.
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MPB59，牛分枝杆菌 BCG 的一种广泛交叉反应蛋白。
DOI：
10.1159/000234154
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
N. Cruz
中科院分区：
文献类型：
--
作者：
H. Wiker;M. Harboe;S. Nagai;M. Patarroyo;C. Ramírez;N. Cruz
从BCG东京亚株培养液中分离纯化了牛分枝杆菌（MycobacteriumbovisBCG）的MPB 59蛋白，并对其进行了生物化学和免疫学鉴定。SDS-聚丙烯酰胺凝胶电泳测定分子量为28，000，pI值为5.3。N-末端氨基酸序列经32步测定，与MPB 64、MPB 70或MPB 80无明显同源性。通过交叉免疫电泳，发现MPB 59属于BCG抗原85复合物，并鉴定为对应于该复合物的85 B组分。该蛋白质与其他种类的分枝杆菌发生广泛的交叉反应，并在接种麻风分枝杆菌后全身分枝杆菌感染的发展过程中诱导了犰狳和猴的显着体液免疫应答。
The MPB59 protein of Mycobacterium bovis BCG was purified to homogeneity from culture fluid of BCG substrain Tokyo, and characterized by biochemical and immunological techniques. The molecular weight was 28,000, determined by SDS-polyacrylamide gel electrophoresis, and the pI value was 5.3. The N-terminal amino acid sequence was determined for 32 steps and showed no significant homology with MPB64, MPB70 or MPB80. By crossed immunoelectrophoresis, MPB59 was found to belong to the BCG antigen 85 complex and identified as corresponding to the 85B component of this complex. The protein cross-reacted extensively with other species of mycobacteria, and induced a marked humoral immune response in armadillos and monkeys during development of systemic mycobacterial infection after inoculation with Mycobacterium leprae.