Selective Near-Infrared Blood Detection Driven by Ionic Liquid–Dye–Albumin Nanointeractions
Selective Near-Infrared Blood Detection Driven by Ionic Liquid–Dye–Albumin Nanointeractions
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由离子液体-染料-白蛋白纳米相互作用驱动的选择性近红外血液检测
DOI：
10.1021/acs.langmuir.3c00727
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发表时间：
2023
期刊：
影响因子：
3.9
通讯作者：
Delcamp, Jared H.
中科院分区：
文献类型：
--
作者：
Darlington, Donovan S.;Mahurin, Allison N.;Kapusta, Karina;Suh, Ember;Smith, Cameron;Jarrett, Ethan;Chism, Claylee M.;Meador, William E.;Kelly, Zakeyia C.;Delcamp, Jared H.
由于其在血液中的丰富性，已经进行了大量的研究来开发用于血清白蛋白的有效生物传感器，并提供对这些生物传感分子和蛋白质之间发生的相互作用的深入了解。近红外（NIR，>700 nm）有机染料已被证明是血清白蛋白的有效生物传感器，但它们的有效性在全血中降低。本文中，它表明，近红外磺酸盐中氮茚供体为基础的方酸染料，SO 3SQ，可以加强作为白蛋白的生物传感器通过添加生物相容性离子液体（IL）。具体地，IL乙醇酸胆碱（1：1）在160 mM的浓度下导致在血液存在下染料的增强的荧光发射（“接通”）。通过DLS，ITC和分子动力学等方法研究了荧光增强的原因。此外，进行荧光测量以观察染料-IL系统对人血清白蛋白（HSA）的色氨酸残基的荧光的影响，以及确定其与白蛋白的表观缔合常数。圆二色性（CD）光谱被用来提供证据，染料IL系统不改变白蛋白或DNA的二级结构。我们的研究结果表明，在IL和血液的存在下，染料的增强的荧光是由于白蛋白中的结合位点的多样化，由IL-染料-白蛋白复合物的相互作用控制。
Due to its abundance in blood, a great deal of research has been undertaken to develop efficient biosensors for serum albumin and provide insight into the interactions that take place between these biosensing molecules and the protein. Near-infrared (NIR, >700 nm) organic dyes have been shown to be effective biosensors of serum albumin, but their effectiveness is diminished in whole blood. Herein, it is shown that an NIR sulfonate indolizine-donor-based squaraine dye, SO3SQ, can be strengthened as a biosensor of albumin through the addition of biocompatible ionic liquids (ILs). Specifically, the IL choline glycolate (1:1), at a concentration of 160 mM, results in the enhanced fluorescence emission (“switch-on”) of the dye in the presence of blood. The origin of the fluorescence enhancement was investigated via methods, including DLS, ITC, and molecular dynamics. Further, fluorescence measurements were conducted to see the impact the dye–IL system had on the fluorescence of the tryptophan residue of human serum albumin (HSA), as well as to determine its apparent association constants in relation to albumin. Circular dichroism (CD) spectroscopy was used to provide evidence that the dye–IL system does not alter the secondary structures of albumin or DNA. Our results suggest that the enhanced fluorescence of the dye in the presence of IL and blood is due to diversification of binding sites in albumin, controlled by the interaction of the IL–dye–albumin complex.