Aptamer conjugated magnetic nanoparticles as nanosurgeons
Aptamer conjugated magnetic nanoparticles as nanosurgeons
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DOI：
10.1088/0957-4484/21/45/455102
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发表时间：
2010-11-12
期刊：
影响因子：
3.5
通讯作者：
Kumar, D. Sakthi
中科院分区：
文献类型：
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作者：
Nair, Baiju G.;Nagaoka, Yutaka;Kumar, D. Sakthi
磁性纳米粒子在靶向给药、热疗和癌症治疗中的磁共振成像（MRI）领域显示出了希望。磁性纳米颗粒进行表面改性的能力以及外部磁场对其运动动力学的影响使其成为癌症破坏的极好纳米平台。由于癌症的高度浸润性，从器官或组织内部选择性地手术切除癌细胞或不需要的细胞而没有任何附带损害是一个严重的问题。为了解决这个问题，在手术中，我们已经开发了一种nanosurgeon的选择性去除靶细胞使用适配体共轭的磁性纳米粒子控制的外部施加的三维旋转磁场。在纳米外科医生的帮助下，我们能够在体外研究中对靶细胞进行手术。LDH和细胞内钙释放测定证实了由于纳米外科医生的作用而导致的癌细胞死亡，这反过来又消除了被去除细胞增殖的可能性。纳米外科医生将是一个有用的工具，在医学领域的选择性手术和细胞操纵研究。此外，该系统可以升级，通过在磁性纳米颗粒上掺入各种靶向特异性配体，从不同组织中选择性去除复杂的癌症。
Magnetic nanoparticles have shown promise in the fields of targeted drug delivery, hyperthermia and magnetic resonance imaging (MRI) in cancer therapy. The ability of magnetic nanoparticles to undergo surface modification and the effect of external magnetic field in the dynamics of their movement make them an excellent nanoplatform for cancer destruction. Surgical removal of cancerous or unwanted cells selectively from the interior of an organ or tissue without any collateral damage is a serious problem due to the highly infiltrative nature of cancer. To address this problem in surgery, we have developed a nanosurgeon for the selective removal of target cells using aptamer conjugated magnetic nanoparticles controlled by an externally applied three-dimensional rotational magnetic field. With the help of the nanosurgeon, we were able to perform surgical actions on target cells in in vitro studies. LDH and intracellular calcium release assay confirmed the death of cancer cells due to the action of the nanosurgeon which in turn nullifies the possibility of proliferation by the removed cells. The nanosurgeon will be a useful tool in the medical field for selective surgery and cell manipulation studies. Additionally, this system could be upgraded for the selective removal of complex cancers from diverse tissues by incorporating various target specific ligands on magnetic nanoparticles.