Effect of GDF-5 on ligament healing
Effect of GDF-5 on ligament healing
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DOI：
10.1002/jor.20002
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Fukui, N
中科院分区：
文献类型：
--
作者：
Tashiro, T;Hiraoka, H;Fukui, N
采用大鼠膝关节内侧副韧带间隙损伤模型，研究了生长分化因子-5(GDF-5)对韧带愈合的影响。手术时一次性应用GDF-5显著改善了股骨-韧带-胫骨复合体的力学性能。术后3周，与载体对照组(P<；0.05；Fisher‘s PLSD试验)相比，30mgGDF-5使复合体的极限拉伸强度提高了41%，硬度提高了60%。透射电子显微镜观察显示，GDF-5增加了修复组织中胶原纤维的直径，这可能是生物力学测试呈阳性结果的机制之一。定量聚合酶链式反应和原位杂交显示GDF-5促进了I型前胶原的表达，而GDF-5处理组的III型前胶原相对I型前胶原表达降低。进一步的聚合酶链式反应分析表明，生长因子对I型前胶原蛋白的表达相对减少，这可能是修复组织中胶原纤维直径增大的原因。没有观察到不良反应，GDF-5的使用被认为是促进韧带愈合的一种有前途的方法。(C)2005年骨科研究会。由威利期刊出版公司出版。
The effects of growth and differentiation factor-5 (GDF-5) on ligament healing were studied using a gap injury model of the medial collateral ligament in rat knee joints. The administration of GDF-5 once at the time of surgery significantly improved the mechanical properties of the femur-ligament-tibia complex. At 3 weeks after surgery, 30 mu g of GDF-5 improved the ultimate tensile strength of the complex by 41%, and the stiffness by 60%, compared with the vehicle control (p < 0.05 for both; Fisher's PLSD test). The observation with a transmission electron microscopy revealed that GDF-5 increased the diameter of collagen fibrils in the repair tissue, which was considered to be a possible mechanism for the positive result in the biomechanical testing. Quantitative PCR and in situ hybridization revealed enhanced type 1 procollagen expression by GDF-5, and the PCR analysis also revealed that the GDF-5 treatment reduced the expression of type III procollagen relative to type I procollagen. The PCR analysis further showed that the expression of decorin and fibromodulin was relatively reduced against type I procollagen by the growth factor, which was considered to be responsible for the increase of collagen fibril diameter in the repair tissue. No adverse effects were observed, and the use of GDF-5 was considered a promising approach to facilitate ligament healing. (c) 2005 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.