Factors regulating template switch in vitro by viral RNA-dependent RNA polymerases:: Implications for RNA-RNA recombination
Factors regulating template switch in vitro by viral RNA-dependent RNA polymerases:: Implications for RNA-RNA recombination
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DOI：
10.1073/pnas.081077198
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发表时间：
2001-04-24
影响因子：
11.1
通讯作者：
Kao, C
中科院分区：
文献类型：
--
作者：
Kim, MJ;Kao, C
复制选择RNA重组发生在病毒RNA合成过程中，当病毒转录复合体切换模板时。我们证明了来自牛病毒性腹泻病毒的RNA依赖的RNA聚合酶和来自三种感染植物的RNA病毒的复制酶可以在体外通过在延长RNA合成过程中切换模板产生易于检测的重组产物。模板序列和/或结构、NTP可用性影响转录复合体切换模板的频率。我们的研究结果为复制选择重组提供了生化支持，并建立了体外分子间RNA重组的机制分析方法。
Copy-choice RNA recombination occurs during viral RNA synthesis when the viral transcription complex switches templates. We demonstrate that RNA-dependent RNA polymerase from bovine Viral diarrhea virus and the replicases from three plant-infecting RNA viruses can produce easily detectable recombination products in vitro by switching templates during elongative RNA synthesis. Template sequence and/or structure, and NTP availability affected the frequency of template switch by the transcription complex. Our results provide biochemical support for copy-choice recombination and establish assays for mechanistic analyses of intermolecular RNA recombination in vitro.