Functional adenosine triphosphate-sensitive potassium channel is required in high-carbohydrate diet-induced increase in β-cell mass.
Functional adenosine triphosphate-sensitive potassium channel is required in high-carbohydrate diet-induced increase in β-cell mass.
复制标题
在高碳水化合物饮食诱导的β细胞质量增加中，需要三磷酸腺苷敏感的钾通道。
DOI：
10.1111/jdi.12907
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发表时间：
2019-03
影响因子：
3.2
通讯作者：
Arima H
中科院分区：
文献类型：
--
作者：
Murase M;Seino Y;Maekawa R;Iida A;Hosokawa K;Hayami T;Tsunekawa S;Hamada Y;Yokoi N;Seino S;Hayashi Y;Arima H
已知高碳水化合物饮食会增加胰岛素分泌并诱导肥胖。然而，高碳水化合物饮食是否会影响β细胞质量（β-cell mass，简写为β-cell mass，简写为B-cell mass）的研究很少。野生型（WT）小鼠和三磷酸腺苷敏感性钾通道缺陷型（Kir6.2KO）小鼠均饲喂正常饲料或高淀粉（ST）饲料22周。通过免疫组化分析胰岛的数量和数量，并通过定量真实的时间逆转录聚合酶链反应研究胰岛中的基因表达水平。MIN 6-K8 β-细胞在含有不同浓度葡萄糖、硝苯地平和格列美脲的溶液中刺激，并分析基因表达。喂食ST的WT和Kir6.2KO小鼠均显示高胰岛素血症和体重增加。与正常饲料喂养的WT小鼠相比，ST喂养的WT小鼠的胰岛数量和cyclinD 2信使核糖核酸的表达水平增加。相比之下，在饲喂正常食物的Kir6.2KO小鼠和饲喂ST的小鼠之间，未观察到胰岛数量或cyclinD 2信使核糖核酸表达水平的显著差异。在高糖培养基中或与格列美脲一起孵育MIN 6-K8 β细胞可增加cyclinD 2表达，而硝苯地平可减弱高糖诱导的cyclinD 2表达增加。这些结果表明，高淀粉饮食以三磷酸腺苷敏感性钾通道依赖性方式增加了胰岛素抵抗，这是通过上调cyclinD 2表达介导的。
A high‐carbohydrate diet is known to increase insulin secretion and induce obesity. However, whether or not a high‐carbohydrate diet affects β‐cell mass (BCM) has been little investigated. Both wild‐type (WT) mice and adenosine triphosphate‐sensitive potassium channel‐deficient (Kir6.2KO) mice were fed normal chow or high‐starch (ST) diets for 22 weeks. BCM and the numbers of islets were analyzed by immunohistochemistry, and gene expression levels in islets were investigated by quantitative real‐time reverse transcription polymerase chain reaction. MIN6‐K8 β‐cells were stimulated in solution containing various concentrations of glucose combined with nifedipine and glimepiride, and gene expression was analyzed. Both WT and Kir6.2KO mice fed ST showed hyperinsulinemia and body weight gain. BCM, the number of islets and the expression levels of cyclinD2 messenger ribonucleic acid were increased in WT mice fed ST compared with those in WT mice fed normal chow. In contrast, no significant difference in BCM, the number of islets or the expression levels of cyclinD2 messenger ribonucleic acid were observed between Kir6.2KO mice fed normal chow and those fed ST. Incubation of MIN6‐K8 β‐cells in high‐glucose media or with glimepiride increased cyclinD2 expression, whereas nifedipine attenuated a high‐glucose‐induced increase in cyclinD2 expression. These results show that a high‐starch diet increases BCM in an adenosine triphosphate‐sensitive potassium channel‐dependent manner, which is mediated through upregulation of cyclinD2 expression.
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