Effects of dietary crude protein and tannic acid on rumen fermentation, rumen microbiota and nutrient digestion in beef cattle
Effects of dietary crude protein and tannic acid on rumen fermentation, rumen microbiota and nutrient digestion in beef cattle
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日粮粗蛋白和单宁酸对肉牛瘤胃发酵、瘤胃微生物及营养物质消化的影响
DOI：
10.1080/1745039x.2018.1545502
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发表时间：
2019-01-01
影响因子：
2
通讯作者：
Zhao, Guangyong
中科院分区：
文献类型：
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作者：
Zhou, Ke;Bao, Yu;Zhao, Guangyong
本试验旨在研究日粮粗蛋白(CP)和单宁酸(TA)对肉牛瘤胃发酵、微生物区系和营养物质消化的影响。选用8头生长肉牛(体重350+/-25 kg)，采用2×2交叉试验设计，试验日粮粗蛋白水平分别为111g/kg干物质(DM)和136g/kg干物质(DM)，TA水平为0和16.9g/kg干物质(DM)。每个实验期为19d，包括14d适应和5d采样。利用16S rRNA基因高通量测序技术分析了日粮粗蛋白和TA对瘤胃微生物区系的影响。结果表明，日粮粗蛋白和TA对瘤胃发酵和养分消化率无交互作用。日粮粗蛋白质水平从111 g/kg DM提高到136g/kg DM时，瘤胃氨氮(NH3-N)浓度升高(p
The objectives of the trial were to study the effects of dietary crude protein (CP) and tannic acid (TA) on rumen fermentation, microbiota and nutrient digestion in beef cattle. Eight growing beef cattle (live weight 350 +/- 25 kg) were allocated in a 2x2 crossover design using two levels of dietary CP [111g/kg dry matter (DM) and 136g/kg DM] and two levels of TA (0 and 16.9g/kg DM) as experimental treatments. Each experimental period lasted 19 d, consisting of 14-d adaptation and 5-d sampling. The impacts of dietary CP and TA on ruminal microbiota were analysed using high-throughput sequencing of 16S rRNA gene. Results indicated that no interactions between dietary CP and TA were found on rumen fermentation and nutrient digestibility. Increasing dietary CP level from 111 to 136g/kg DM increased the ruminal concentrations of ammonia nitrogen (NH3-N) (p