A subset of yeast snRNA's contains functional binding sites for the highly conserved Sm antigen.
A subset of yeast snRNA's contains functional binding sites for the highly conserved Sm antigen.
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酵母 snRNA 的一个子集包含高度保守的 Sm 抗原的功能结合位点。
DOI：
10.1126/science.2948278
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Guthrie,C
中科院分区：
文献类型：
--
作者：
Riedel,N;Wolin,S;Guthrie,C
Sm特异性的自身免疫血清能与昆虫和鞭毛虫等进化上存在差异的生物体中含有小核RNA （snRNA）的核糖核蛋白颗粒（snRNP's）发生反应，但据报道不能识别来自酵母的snRNP's。Sm抗原被认为与一个保守的snRNA基序结合，包括序列a (U3-6)G。这一假设得到了验证，酵母也含有Sm snRNA的功能类似物，但RNA中的Sm结合位点比Sm抗原决定因子更为严格保守。在显微注射标记的酵母snRNA后，酿酒酵母的两种snRNA与人类自身抗体（anti-Sm）具有很强的免疫沉淀性。它们每个都包含序列A(U5-6)G，对生存至关重要，并且是剪接体的组成部分。至少有六种其他酵母snRNA不具有免疫沉淀性，并且缺乏该序列；这些非sm snRNA都是可有可无的。
Autoimmune sera of the Sm specificity react with the major class of small nuclear RNA (snRNA)-containing ribonucleoprotein particles (snRNP's) from organisms as evolutionarily divergent as insects and dinoflagellates but have been reported not to recognize snRNP's from yeast. The Sm antigen is thought to bind to a conserved snRNA motif that includes the sequence A(U3-6)G. The hypothesis was tested that yeast also contains functional analogs of Sm snRNA's, but that the Sm binding site in the RNA is more strictly conserved than the Sm antigenic determinant. After microinjection of labeled yeast snRNA's intoXenopuseggs or oocytes, two snRNA's fromSaccharomyces cerevisiaebecome strongly immunoprecipitable with human auto-antibodies known as anti-Sm. These each contain the sequence A(U5-6)G, are essential for viability, and are constituents of the spliceosome. At least six other yeast snRNA's do not become immunoprecipitable and lack this sequence; these non-Sm snRNA's are all dispensable.