The dynamin-related GTPase, Dnm1p, controls mitochondrial morphology in yeast.
The dynamin-related GTPase, Dnm1p, controls mitochondrial morphology in yeast.
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DOI：
10.1083/jcb.143.2.333
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发表时间：
1998-10-19
期刊：
影响因子：
--
通讯作者：
Shaw JM
中科院分区：
文献类型：
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作者：
Otsuga D;Keegan BR;Brisch E;Thatcher JW;Hermann GJ;Bleazard W;Shaw JM
酿酒酵母Dnm1蛋白在结构上与动力蛋白相关，动力蛋白是胞吞过程中膜分裂所需的GTPase。在这里，我们表明Dnm1p对线粒体形态的维持至关重要。DNM1基因的破坏导致野生型管状线粒体膜网络塌陷到细胞的一侧，但不影响其他细胞器的形态或分布。在预测的gtp结合区域中含有点突变或完全缺乏gtp结合区域的Dnm1蛋白不能挽救Dnm1突变体中的线粒体形态缺陷，并在野生型细胞中诱导显性线粒体形态缺陷。间接免疫荧光显示Dnm1p分布在与线粒体室共定位的细胞皮层的点状结构中。这些含有dnm1p的结构仍然与在mdm10突变株中发现的球形线粒体有关。此外，在野生型细胞的差异沉淀和蔗糖梯度分离过程中，一部分Dnm1p与线粒体膜分离。我们的研究结果表明，Dnm1p是酵母线粒体网络皮层分布所必需的，这是一种动力蛋白相关蛋白的新功能。
The Saccharomyces cerevisiae Dnm1 protein is structurally related to dynamin, a GTPase required for membrane scission during endocytosis. Here we show that Dnm1p is essential for the maintenance of mitochondrial morphology. Disruption of the DNM1 gene causes the wild-type network of tubular mitochondrial membranes to collapse to one side of the cell but does not affect the morphology or distribution of other cytoplasmic organelles. Dnm1 proteins containing point mutations in the predicted GTP-binding domain or completely lacking the GTP-binding domain fail to rescue mitochondrial morphology defects in a dnm1 mutant and induce dominant mitochondrial morphology defects in wild-type cells. Indirect immunofluorescence reveals that Dnm1p is distributed in punctate structures at the cell cortex that colocalize with the mitochondrial compartment. These Dnm1p-containing structures remain associated with the spherical mitochondria found in an mdm10 mutant strain. In addition, a portion of Dnm1p cofractionates with mitochondrial membranes during differential sedimentation and sucrose gradient fractionation of wild-type cells. Our results demonstrate that Dnm1p is required for the cortical distribution of the mitochondrial network in yeast, a novel function for a dynamin-related protein.