Cultivation of Chlorella pyrenoidosa in anaerobic wastewater: The coupled effects of ammonium, temperature and pH conditions on lipids compositions
Cultivation of Chlorella pyrenoidosa in anaerobic wastewater: The coupled effects of ammonium, temperature and pH conditions on lipids compositions
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厌氧废水中蛋白核小球藻的培养：铵、温度和 pH 条件对脂质成分的耦合影响
DOI：
10.1016/j.biortech.2019.03.117
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发表时间：
2019
影响因子：
11.4
通讯作者：
Li Xiao Yong
中科院分区：
文献类型：
--
作者：
Zhao Xian Chao;Tan Xiao Bo;Yang Li Bin;Liao Jian Yu;Li Xiao Yong
厌氧废水是培养微藻的理想培养基。氨氮、温度和pH对藻类油脂积累的耦合作用是厌氧培养藻类过程中的核心问题。因此，研究了它们对蛋白核小球藻培养和厌氧出水中脂质积累的联合作用。pH和温度升高诱导的游离氨抑制藻类生长，但显著促进脂质积累。在pH值为8.3-8.5（25 °C，铵280 mg/L）时，脂肪含量最高，达到30.2%，是中性条件下的1.6倍。pH值为8.3-8.5时，不饱和脂肪酸（un-SFA）含量增加到74.8-77.9%，而中性条件下只有56.1-58.9%。在高pH条件下，C18：2和C18：3占主要地位，C18：3的比例增加了74.5- 153.1%。本研究为利用厌氧废水培养藻类积累油脂提供了一种可能的途径。
Anaerobic wastewater potentially was an ideal medium for cultivating microalgae. The coupled effect of ammonium, temperature and pH on lipids accumulation was a core issue during algal culture using anaerobic wastewater. Therefore, their combined effects onChlorella pyrenoidosaculture and lipids accumulation in anaerobic effluent were investigated. Free ammonia induced from the rising pH and temperature inhibited algal growth, but significantly promoted lipid accumulation. The highest lipids content reached 30.2% when pH rose to 8.3–8.5 (25 °C, ammonium 280 mg/L), which was 1.6-fold higher than that under neutral condition. Moreover, the percentage of unsaturated fatty acids (un-SFAs) increased to 74.8–77.9% at pH 8.3–8.5, whereas it was only 56.1–58.9% under neutral condition. The C18:2 and C18:3 dominated the un-SFAs increase at high pH, typically the percentage of C18:3 increased by 74.5–153.1%. This study provides a potential way for lipid accumulation in algal culture using anaerobic wastewater.