Enhanced osteogenic differentiation of MC3T3-E1 on rhBMP-2-immobilized titanium via click reaction
Enhanced osteogenic differentiation of MC3T3-E1 on rhBMP-2-immobilized titanium via click reaction
复制标题
DOI：
10.1016/j.carbpol.2013.12.023
复制
发表时间：
2014-03-15
影响因子：
11.2
通讯作者：
Lee, Deok-Won
中科院分区：
文献类型：
--
作者：
Kim, Eun-Cheol;Kim, Tae-Hee;Lee, Deok-Won
在本研究中，我们报道了骨形态发生蛋白-2(BMP-2)通过点击反应固定在钛表面促进MC3T3-E1细胞成骨分化的效果。用静态接触角和XPS对其表面进行了表征，结果表明，通过点击化学方法对纯钛(Ti-1)进行了表面修饰。通过对氨化钛(Ti-4)固定化肝素的定量分析，发现Ti-4可以作为固定化肝素等生物分子的良好候选材料。用骨形态发生蛋白-2(钛-6)固定的钛的骨形态发生蛋白-2持续释放28d。MC3T3-E1细胞在Ti-6上的增殖率最高。通过体外碱性磷酸酶(ALP)活性、钙沉积和实时聚合酶链式反应(Real-time PCR)检测，我们发现钛-6可以作为一种良好的植入材料来促进MC3T3-E1细胞的成骨分化。(C)2013爱思唯尔有限公司。保留所有权利。
In the present study, we report about the efficacy of titanium surface-immobilized with bone morphogenetic protein-2 (BMP-2) via click reaction on enhanced osteogenic differentiation of MC3T3-E1 cells. The surface was characterized by static contact angles and XPS measurements, which indicated that pristine titanium (Ti-1) was successfully surface-modified via click chemistry (aminated titanium, Ti-4). By quantitative analysis of heparin immobilized on aminated titanium (Ti-4), we found that the Ti-4 can be used as a good candidate to immobilize biomolecules such as heparin. BMP-2 from titanium immobilized with BMP-2 (Ti-6) was released for a period of 28 days in a sustained manner. The highest proliferation rate of MC3T3-E1 cells was observed on Ti-6. Through in vitro tests including alkaline phosphatase (ALP) activity, calcium deposition and real-time polymerase chain reaction (real-time PCR), we found that Ti-6 can be used as a good implant to enhance the osteogenic differentiation of MC3T3-E1 cells. (C) 2013 Elsevier Ltd. All rights reserved.