Enzyme-guided plasmonic biosensor based on dual-functional nanohybrid for sensitive detection of thrombin
Enzyme-guided plasmonic biosensor based on dual-functional nanohybrid for sensitive detection of thrombin
复制标题
基于双功能纳米杂化物的酶引导等离子体生物传感器用于凝血酶的灵敏检测
DOI：
10.1016/j.bios.2015.03.024
复制
发表时间：
2015
影响因子：
12.6
通讯作者：
Li Jinghong
中科院分区：
文献类型：
--
作者：
Yan Jing;Wang Lida;Tang Longhua;Lin Lei;Liu Yang;Li Jinghong
快速、灵敏的蛋白质检测方法是临床诊断的迫切需求。金属纳米结构的局域表面等离子体共振（LSPR）由于其灵敏的光学性质和强的电磁近场增强而有可能避免这个问题。在这项工作中，酶介导的等离子体生物传感器的基础上的双功能纳米杂化物的凝血酶的检测。通过利用LSPR响应性纳米杂交体和适配体-酶缀合的报告探针，传感平台带来增强的信号、稳定性以及简单性。酶促反应催化Au 3+原位还原为Au°，进一步导致金纳米颗粒（AuNPs）的快速晶体生长。随着纳米粒子的生成，LSPR的吸收带和颜色发生了变化，可以通过紫外可见分光光度计和肉眼进行实时监测。由金和磁性纳米粒子构建的纳米杂化物作为双功能等离子体单元，既起到信号产生的作用，又赋予传感器磁分离的功能。同时，酶的引入有效地调节了AuNPs的程序化晶体生长。此外，由于酶的高催化效率，它还可以作为信号放大器。等离子体传感器的响应随对数凝血酶浓度线性变化，直至10 nM，检测限为200 pM。所提出的策略显示出良好的分析性能的凝血酶测定。这种简单的一次性方法在开发用于蛋白质监测、药物发现和即时诊断的通用平台方面很有前途。
Rapid and sensitive methodologies for the detection of protein are in urgent requirement for clinic diagnostics. Localized surface plasmon resonance (LSPR) of metal nanostructures has the potential to circumvent this problem due to its sensitive optical properties and strong electromagnetic near-field enhancements. In this work, an enzyme mediated plasmonic biosensor on the basis of a dual-functional nanohybrid was developed for the detection of thrombin. By utilizing LSPR-responsive nanohybrid and anaptamer-enzyme conjugated reporting probe, the sensing platform brings enhanced signal,stability as well as simplicity. Enzymatic reaction catalyzed the reduction of Au3+to Au°in situ, further leading to the rapid crystal growth of gold nanoparticles (AuNPs). The LSPR absorbance band and color changed company with the nanoparticle generation, which can be real-time monitoring by UV–visible spectrophotometer and naked eye. Nanohybrid constructed by gold and magnetic nanoparticles acts as a dual functional plasmonic unit, which not only plays the role of signal production, but also endows the sensor with the function of magnetic separation. Simultaneously, the introduction of enzyme effectively regulates the programming crystal growth of AuNPs. In addition, enzyme also serves as signal amplifier owing to its high catalysis efficiency. The response of the plasmonic sensor varies linearly with the logarithmic thrombin concentration up to 10 nM with a limit of detection of 200 pM. The as-proposed strategy shows good analytical performance for thrombin determination. This simple, disposable method is promising in developing universal platforms for protein monitoring, drug discovery and point-of-care diagnostics.