Osteogenic potential of rat mesenchymal stem cells after several passages
Osteogenic potential of rat mesenchymal stem cells after several passages
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DOI：
10.1016/j.bbrc.2004.02.038
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发表时间：
2004-03-26
影响因子：
3.1
通讯作者：
Ishiguro, N
中科院分区：
文献类型：
--
作者：
Sugiura, F;Kitoh, H;Ishiguro, N
对连续传代的大鼠骨髓间充质干细胞(BMCs)的成骨能力进行临床可行性评价。通过碱性磷酸酶和骨钙素的浓度和基因表达来评价体外成骨分化。为了体内成骨，将不同分化程度的骨髓基质细胞植入裸鼠体内。尽管成骨标志物的水平在连续培养的传代较少的骨髓细胞中显著升高(OS组)，但随着传代的进行，成骨标志物的水平降低。与体外实验相似，OS组PO~P2细胞膜内可见丰富的骨和软骨形成。然而，在P3细胞中，腔内充满了纤维组织，表明成骨失败。为了将来的临床应用，需要建立允许骨髓基质细胞快速扩增的培养条件，同时保持其分化的潜力。(C)2004 Elsevier Inc.保留所有权利。
Osteogenic potential of serially passaged rat bone marrow derived mesenchymal stem cells (BMCs) was evaluated for clinical feasibility. Osteogenic differentiation in vitro was evaluated by means of the concentration and mRNA expression of alkaline phosphatase and osteocalcin. For in vivo osteogenesis, BMCs in various degrees of differentiation were implanted into the athymic mice. Although elevated levels of osteogenic markers were prominent in the less passaged BMCs continuously cultured with osteogenic supplements (OS group), they decreased with passaging. Similar to the in vitro experiments, abundant bone and cartilage formations inside the membrane were observed in the PO through P2 cells of the OS group. In the P3 cells, however, the chambers were filled with fibrous tissues showing the failure of osteogenesis. Establishment of the culture conditions that permit the rapid expansion of BMCs while retaining their potential for differentiation will be required for future clinical applications. (C) 2004 Elsevier Inc. All rights reserved.