Effects of bilateral striatal 6-OHDA lesions on circadian rhythms in the rat - A radiotelemetric study
Effects of bilateral striatal 6-OHDA lesions on circadian rhythms in the rat - A radiotelemetric study
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DOI：
10.1016/s0024-3205(00)00751-7
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发表时间：
2000-08-18
期刊：
影响因子：
6.1
通讯作者：
Bruguerolle, B
中科院分区：
文献类型：
--
作者：
Ben, V;Bruguerolle, B
目前的工作旨在记录，在先前描述的帕金森动物模型（双侧纹状体注射 6-OHDA）中，通过遥测连续记录的昼夜节律标记节律，即温度（T）、心率（H）和运动活动（A）的可能修改，以评估昼夜节律的可能扰动。 H、T 和 A 在发射器手术植入后通过无线电遥测测量（数据科学）。恢复期后，该研究被分为控制期 (C)，其中包括 T、H 和 A 日节律的基线测量。然后对4只大鼠进行立体定向6-OHDA纹状体损伤，对照组大鼠纹状体注射生理盐水；观察第四个登记周的第二阶段（D 1-7 (W1)、D 8-14 (W2)、D 15-21 (W3)、D 22-28 (W4)}。最后，在该阶段结束时，将七只大鼠斩首以确定其纹状体多巴胺 (DA) 含量。我们的数据记录了 H、T 和 A 的昼夜节律根据时间受到不同的影响。因此，暂时损失还观察到 6-OHDA 引起的 H、T 和 A 昼夜节律特征的改变：观察到三种节律的中值显着降低以及这些节律的振幅，在干预后的四个星期内观察到这些扰动，但从未达到初始控制水平。抗帕金森病药物的时间药理学 (C) 2000 Elsevier Science Inc. 保留所有权利。
The present work aims to document, in a previously described animal model of Parkinson (double bilateral striatal injection of 6-OHDA), the possible modifications of circadian markers rhythms i.e. temperature (T), heart rate (H) and locomotor activity (A) registered continuously by telemetry, in order to evaluate a possible perturbation of the circadian rythmicity. H, T and A were measured by radiotelemetry after surgical implantation of the transmitters (Data Sciences). After a recovery period, the study was divided into a control period (C) fur baseline measurements of T, H and A daily rhythms. Then, the stereotaxic 6-OHDA striatal lesion was done to a group of 4 rats while the control rats were injected into striata with saline; a second period of four registration weeks was observed (D 1-7 (W1), D 8-14 (W2), D 15-21 (W3), D 22-28 (W4)}. Finally, at the end of this period the seven rats were decapited in order to determine their striatal dopamine (DA) content. Our data document that the circadian rhythms of H,T and A were differently affected according to time. Thus, a temporary loss of circadian periodicity was observed particularly for heart rate. 6-OHDA-induced modifications of H, T and A circadian rhythm characteristics were also observed: a significant decrease of the mesor was observed for the three rhythms as well as a phase advance. Concerning the amplitude of these rhythms, only H was significantly decreased. These perturbations were observed during the four weeks following the intervention, never reaching the initial control levels. Such observed perturbations would supply a basis for the future study of the chronopharmacology of antiparkinsonian drugs. (C) 2000 Elsevier Science Inc. All rights reserved.