Transplantation of Co-aggregates of Sertoli Cells and Islet Cells into Liver Without Immunosuppression.
Transplantation of Co-aggregates of Sertoli Cells and Islet Cells into Liver Without Immunosuppression.
复制标题
将支持细胞和胰岛细胞的共聚集体移植到肝脏中，无需免疫抑制。
DOI：
10.1097/01.tp.0000438198.76531.03
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发表时间：
2014
期刊：
影响因子：
6.2
通讯作者：
and Hiroo Iwata
中科院分区：
文献类型：
--
作者：
Naohiro Takemoto;Xibao Liu;Kento Takii;Yuji Teramura;and Hiroo Iwata
背景胰岛移植用于治疗胰岛素依赖型糖尿病。然而，胰岛移植物必须通过施用免疫抑制药物来维持，这可能导致长期并发症。一种方法，避免了免疫抑制药物的使用是可取的。MethodsCo聚集体的支持细胞和胰岛细胞从BALB/c小鼠，由悬滴法制备的移植到C57 BL/6小鼠的肝脏通过门静脉在人类临床胰岛transplantation.ResultsThe核心部分的聚集体主要包含支持细胞，这些细胞被胰岛细胞包围。共聚集体保留了支持细胞和胰岛细胞的功能。当800个共聚集体通过门静脉移植到7只C57 BL/6小鼠中时，7只受体小鼠中有6只表现出超过100 day.ConclusionsThe悬滴法适用于制备用于移植的Sertoli和胰岛细胞聚集体。值得注意的是，通过门静脉将这些同种异体共聚集体移植到患有化学诱导的糖尿病的小鼠中导致了长期移植物存活而没有全身免疫抑制。
BackgroundTransplantation of islets of Langerhans (islets) was used to treat insulin-dependent diabetes mellitus. However, islet grafts must be maintained by administration of immunosuppressive drugs, which can lead to complications in the long term. An approach that avoids immunosuppressive drug use is desirable.MethodsCo-aggregates of Sertoli cells and islet cells from BALB/c mice that were prepared by the hanging drop method were transplanted into C57BL/6 mouse liver through the portal vein as in human clinical islet transplantation.ResultsThe core part of the aggregates contained mainly Sertoli cells, and these cells were surrounded by islet cells. The co-aggregates retained the functions of both Sertoli and islet cells. When 800 co-aggregates were transplanted into seven C57BL/6 mice via the portal vein, six of seven recipient mice demonstrated quasi-normoglycemia for more than 100 days.ConclusionsThe hanging drop method is suitable for preparing aggregates of Sertoli and islet cells for transplantation. Notably, transplantation of these allogeneic co-aggregates into mice with chemically induced diabetes via the portal vein resulted in long-term graft survival without systemic immunosuppression.