clifford (B.4.1) , an allele of CG1603 , causes tissue overgrowth in the Drosophila melanogaster eye.
clifford (B.4.1) , an allele of CG1603 , causes tissue overgrowth in the Drosophila melanogaster eye.
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DOI：
10.17912/micropub.biology.000936
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发表时间：
2023
影响因子：
--
通讯作者：
Merkle, Julie A
中科院分区：
文献类型：
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作者：
Nowaskie, Reagan R;Kitch, Ashley;Adams, Abby;Anandaraj, Abinaya;Apawan, Ethan;Banuelos, Liliana;Betz, Cassandra J;Bogunia, Julia M;Buechlein, Nicholas;Burns, Morgan R;Collier, Hayley A;Collins, Zach;Combs, Kynzie;Dakarian, Vana D;Daniel, Abigail;De Jesus Iii, Conrad M;Erickson, John D;Estrada, Bianca;Estrada, Kevin;Fields, Sydney;Gabriel, Maya;Garcia, Rosario M;Gitamo, Sylvia;Granath, Emma;Hardin, Sabrina N;Hattling, Emily;Henriquez, Alexandra Vl;Hernandez, Destiny;Johnson, Luke;Kim, Annie H;Kolley, Lillian K;Larue, Katelynn M;Lockwood, Erin;Longoria, Nelia;Lopez, Cassandra;Lopez-Roca Fernandez, Rosario C;Lozano, Sofia;Manthie, Carissa;May, Trinity;Mehrzad, Zorah;Mendoza, Itzel;Mohan, Somya;Mounthachak, Claylan;Muyizere, Merveille;Myers, Margaret R;Newton, Jayce;Nwawueze, Amarachi;Paredes, Ariana J;Pezdek, Marissa N;Phat Nguyen, Hoang;Pobuda, Nadia;Sadat, Sahar;Sailor, Johnathon J;Santiago, David;Sbarbaro, Madison;Schultz Iii, David E;Senobari, Anahita N;Shouse, Emma M;Snarski, Sarah M;Solano, Estefanie;Solis Campos, Naomi;Stewart, Elnora;Szczepaniak, Jessica;Tejeda, Michael;Teoli, Dominic F;Tran, Michael;Trivedi, Nishita;Uribe Aristizabal, Laurita;Vargas, Bryan Z;Walker Iii, Kenneth W;Wasiqi, Joseph;Wong, Joyi;Zachrel, Adira;Shah, Hemin P;Small, Elizabeth;Watts, Charlie T;Croonquist, Paula;Devergne, Olivier;Jones, Amy K;Taylor, Elizabeth E;Kagey, Jacob D;Merkle, Julie A
突变体B.4.1，通过EMS诱变产生的果蝇，进行了研究的本科生参与的Fly-CURE。在成人眼睛中诱导遗传镶嵌组织后，B.4.1突变组织显示出细胞分裂的强烈增加和粗糙的外观。互补定位和序列分析确定了基因CG 1603中的无义突变，我们将其命名为clifford（cliff），因为与对照组相比，观察到红色突变组织增加。Cliff编码涉及转录控制的含锌指蛋白。Cliff的RNAi敲除同样导致粗糙的眼睛，证实了Cliff在眼睛发育中的作用。
Mutant B.4.1 , generated via EMS mutagenesis in Drosophila melanogaster , was studied by undergraduate students participating in the Fly-CURE. After inducing genetically mosaic tissue in the adult eye, B.4.1 mutant tissue displays a robust increase in cell division and a rough appearance. Complementation mapping and sequence analysis identified a nonsense mutation in the gene CG1603 , which we named clifford ( cliff ) due to observed increases in red-pigmented mutant tissue compared to controls. cliff encodes a zinc finger-containing protein implicated in transcriptional control. RNAi knockdown of cliff similarly results in rough eyes, confirming a role for Cliff in eye development.