Approaches for eliminating PCR inhibitors and designing PCR primers for the detection of phytopathogenic fungi
Approaches for eliminating PCR inhibitors and designing PCR primers for the detection of phytopathogenic fungi
复制标题
消除 PCR 抑制剂和设计用于检测植物病原真菌的 PCR 引物的方法
DOI：
10.1016/j.cropro.2006.04.014
复制
发表时间：
2007-02-01
期刊：
影响因子：
2.8
通讯作者：
Michailides, Themis J.
中科院分区：
文献类型：
--
作者：
Ma, Zhonghua;Michailides, Themis J.
聚合酶链反应（PCR）技术已被用于快速检测，表征和鉴定各种生物体。在PCR诊断研究中，PCR引物的开发是最重要的步骤之一。本文综述了几种主要的方法，已成功地用于设计特异性PCR引物对各种植物病原真菌。这些方法包括使用物种特异性基因或DNA区域，或匿名的独特DNA区域来设计PCR引物。由于PCR抑制剂的问题是普遍存在的PCR诊断植物病害，我们还审查了各种技术，已被用来规避PCR抑制剂来自植物组织，土壤，空气和水样品。(c)2006爱思唯尔有限公司保留所有权利。
The polymerase chain reaction (PCR) technology has been used to rapidly detect, characterize, and identify a variety of organisms. In a PCR diagnosis study, the development of PCR primers is one of the most important steps. This paper reviews several major approaches that have been used successfully for designing PCR primers specific to various phytopathogenic fungi. These approaches include using species-specific genes or DNA regions, or anonymous unique DNA regions to design PCR primers. Since the problem with PCR inhibitors is prevalent in PCR diagnosis of plant diseases, we also review various techniques that have been used to circumvent PCR inhibitors derived from plant tissues, soil, air, and water samples. (c) 2006 Elsevier Ltd. All rights reserved.