Release of TNF-alpha and IL-1beta from porcine brain endothelium corresponds to the pyrogenic potential of three marketed formulations of amphotericin.
Release of TNF-alpha and IL-1beta from porcine brain endothelium corresponds to the pyrogenic potential of three marketed formulations of amphotericin.
复制标题
猪脑内皮细胞释放的 TNF-α 和 IL-1β 与三种市售两性霉素制剂的致热潜力相对应。
DOI：
10.1007/s00011-005-1370-9
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Miller,DW
中科院分区：
文献类型：
--
作者：
McGuire,TR;Trickler,WJ;Smith,L;Hoie,EB;Miller,DW
背景：阿替西霉素的制剂包括脱氧胆酸盐混悬液（d-Amph）、阿替西霉素-B脂质复合物（AMARC）和脂质体产品（L-Amph）。目的：探讨脑血管内皮细胞释放肿瘤坏死因子-α（TNF-α）和白细胞介素-1-β（IL-1）是否与阿霉素引起的发热有关。NF-κB是TNF-α和IL-1β基因的转录调节因子，其表达与这种分泌模式相对应。TNF-α在治疗后2 h即显著升高（p = 0.0021），而IL-1β在治疗后6 h才显著升高（p = 0.0009）。这些实验还表明，阿替霉素发热最初是由TNF-α介导的。
Background:Formulations of amphotericin include a deoxycholate suspension (d-Amph), an amphotericin-B lipid complex (Ablc), and a liposomal product (L-Amph). Fever is most frequent with d-Amph, intermediate with Ablc, and lowest with L-Amph.Objective:To determine if the release of tumor necrosis factor-alpha (TNF-α) and interleukin-1-beta (IL-1) from brain endothelium corresponds to the incidence of amphotericin fever.Results:Release of TNF-α and IL-1β after L-Amph treatment was similar to negative controls while after d-Amph treatment release was similar to lipopolysaccharide. Ablc treatment produced intermediate pyrogen release.NF-κB expression, a transcriptional regulator for TNF-α and IL-1β genes, corresponded to this secretion pattern. TNF-α release was elevated 2 hours (p = 0.0021) after treatment while significant elevations in IL-1β required 6 hours (p = 0.0009).Conclusion:Results from thisin vitrostudy suggest that amphotericin fever may be directly mediated by brain endothelium. These experiments also suggest that amphotericin fever is initially mediated by TNF-α.