Characterizing the gene mutations associated with resistance to gatifloxacin in Mycobacterium tuberculosis through whole genome sequence.

Characterizing the gene mutations associated with resistance to gatifloxacin in Mycobacterium tuberculosis through whole genome sequence.
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DOI：
10.1016/j.ijid.2021.09.028
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发表时间：
2021-09
期刊：
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
影响因子：
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通讯作者：
Jing Bi;Qinglong Guo;Xiangdong Fu;Juan Liang;Lidong Zeng;M. Ou;Juanjuan Zhang;Zhaoqin Wang;Yicheng Sun;Lei Liu;Guoliang Zhang
Jing Bi;Qinglong Guo;Xiangdong Fu;Juan Liang;Lidong Zeng;M. Ou;Juanjuan Zhang;Zhaoqin Wang;Yicheng Sun;Lei Liu;Guoliang Zhang
中科院分区：
其他
文献类型：
--
作者：
Jing Bi;Qinglong Guo;Xiangdong Fu;Juan Liang;Lidong Zeng;M. Ou;Juanjuan Zhang;Zhaoqin Wang;Yicheng Sun;Lei Liu;Guoliang Zhang

文献摘要

相似文献

ObjectivesGatifloxacin (GAT), a fourth-generation fluoroquinolone (FQ), is used to treat drug-resistant tuberculosis. Although DNA gyrase mutations are the leading cause of FQ resistance, mutations conferring resistance to GAT remain inadequately characterized.MethodsGAT-resistant mutants were selected from 7H10 agar plates containing 0.5 mg/L GAT (critical concentration). Mutations involved in GAT resistance were identified through whole-genome sequencing.ResultsIn total, 123 isolates demonstrated resistance to GAT. Among these isolates, 55.3% (68/123) hadgyrAgene mutations [G280A (D94N), A281G (D94G), G280T (D94Y) and G262T (G88C)]. The remainder (44.7%, 55/123) harbouredgyrBgene mutations [A1495G (N499D), C1497A (N499K), C1497G (N499K) and A1503C (E501D)].ConclusionsMutations in thegyrAandgyrBgenes are the main mechanisms of GAT resistance. These findings provide new insight into GAT resistance, and contribute to molecular diagnosis of GAT resistance in the clinical setting.