One-pot native chemical ligation by combination of two orthogonal thioester precursors
One-pot native chemical ligation by combination of two orthogonal thioester precursors
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DOI：
10.1039/c6cc10243c
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发表时间：
2017-02-14
影响因子：
4.9
通讯作者：
Hojo, Hironobu
中科院分区：
文献类型：
--
作者：
Asahina, Yuya;Kawakami, Toru;Hojo, Hironobu
我们开发了一种一锅肽连接方法，使用两个正交硫酯前体和一个保护基进行 Asp 和 Cys 之间的连接反应。两种前体的组合促进了一锅法操作并产生了完整的多肽。组蛋白 H4 的全合成成功证明了该方法的有效性。
We developed a one-pot peptide ligation method using two orthogonal thioester precursors and a protecting group for the ligation reaction between Asp and Cys. Combination of the two precursors facilitated the one-pot operation and yielded the entire polypeptide. The usefulness of this method was successfully demonstrated by the total synthesis of histone H4.