Experimental studies on the ciliary action of amphibian embryos

Experimental studies on the ciliary action of amphibian embryos
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两栖动物胚胎纤毛作用的实验研究

DOI:
10.1002/jez.1400500302
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发表时间:
1928
影响因子:
--
通讯作者:
V. Twitty
V. Twitty
中科院分区:
--
文献类型:
--
作者:
V. Twitty

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两栖动物胚胎外胚层上活跃纤毛的存在,通过胚胎在卵囊内的旋转而被熟悉地证明。Assheton('96)和Woerdeman(' 25)描述了这些纤毛在正常条件下的行为;前者描述了蛙和海卫一,后者描述了蛙和黑蝾螈。纤毛在神经褶皱闭合时首次出现,其作用指向大部分胚胎的尾部,在水中产生相应的水流,或将胚胎从其囊中取出时沿培养皿向前沿着。本研究主要涉及纤毛作用对实验条件的某些反应,主要是在点钝口鱼胚胎上进行的。在简要叙述了这种形式的正常活动之后,将在以下标题下处理实验结果:纤毛细胞极性的测定;纤毛作用的逆转;箭毒对纤毛作用的影响;以及纤毛作用的协调。
The presence of active cilia on the ectoderm of amphibian embryos is familiarly manifested by the rotation of the embryos within the egg capsules. The behavior of these cilia under normal conditions has been described by Assheton ('96) and by Woerdeman ('25); by the former in Rana temporaria and Triton cristatus, by the latter in Rana esculenta and the black axolotl. First appearing about the time the neural folds close, the cilia have their action directed caudally on most of the embryo, creating a corresponding current in the water, or propelling the embryo anteriorly along the dish when removed from its capsule.The present study,'concerned primarily with certain responses of the ciliary action to experimental situations, was made chiefly on embryos of Amblystoma punctatum. After a brief account of the normal activity in this form, the experimental results will be treated under the following headings: determination of polarity of ciliated cells; reversal of ciliary action; effect of curare on ciliary action; and coordination of ciliary action.