Measuring pH of the Coxiella burnetii Parasitophorous Vacuole.
Measuring pH of the Coxiella burnetii Parasitophorous Vacuole.
复制标题
测量伯氏柯克斯体寄生液泡的 pH 值。
DOI：
10.1002/cpmc.38
复制
发表时间：
2017
影响因子：
--
通讯作者：
Gilk,StaceyD
中科院分区：
文献类型：
--
作者：
Samanta,Dhritiman;Gilk,StaceyD
The gram‐negative bacteriumCoxiella burnetiicauses human Q fever, a disease characterized by a debilitating flu‐like illness in acute cases and endocarditis in chronic patients. An obligate intracellular pathogen,Coxiella burnetiisurvives within a large, lysosome‐like vacuole inside the host cell. A unique feature of theCoxiellaparasitophorous vacuole (PV) is high levels of fusion with the host endocytic pathway, with PV‐endosome fusion critical forCoxiellasurvival within the host cell. This unit describes quantitating PV‐endosome fusion by measuring delivery of the fluid phase endosome marker dextran to the PV using live cell imaging. To study the effect of host cell proteins involved in PV‐endosome fusion, details are provided for using siRNA knockdown host cells. This method is a powerful tool for understanding mechanisms underlyingCoxiella’s ability to manipulate host cell trafficking pathways. © 2017 by John Wiley & Sons, Inc.
