INTERSPECIFIC HYBRIDIZATION BETWEEN ASPERGILLUS-NIDULANS AND ASPERGILLUS-RUGULOSUS BY FUSION OF SOMATIC PROTOPLASTS
INTERSPECIFIC HYBRIDIZATION BETWEEN ASPERGILLUS-NIDULANS AND ASPERGILLUS-RUGULOSUS BY FUSION OF SOMATIC PROTOPLASTS
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DOI：
10.1099/00221287-102-2-255
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
PEBERDY, JF
中科院分区：
文献类型：
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作者：
KEVEI, F;PEBERDY, JF
利用聚乙二醇诱导体细胞原生质体融合的方法，在褐花拟南芥（A. nidulans）和褐花拟南芥（A. rugulosus）的营养缺陷突变体之间产生了种间异核体。异核体生长非常缓慢，产生不规则形状的菌落，在完全培养基上，亲本菌株很容易分离。在最小培养基上的长期培养过程中，许多异核体产生了旺盛生长的部分，其特征是分泌棕色色素，并且在完全培养基上继代培养时具有稳定性。从异核体分离的原生质体在最小培养基上再生时产生相同的新集落类型，在完全培养基上再生时产生新的集落类型和亲本类型。推测新菌落为种间杂交，其营养形态正常，菌落形状和大小规则，但产生新的分生孢子。在杂交发育早期形成的分生孢子产生了杂交菌落。观察到大小不一的分生孢子；最大的为无核，最小的为无核。杂种和亲本的分生孢子大小、细胞核数和每核DNA含量的比较表明，杂种为二倍体。杂种老培养的分生孢子具有异质性，萌发时出现杂交、亲本和重组菌落类型。在苯甲酰存在的情况下培养杂种，亲本和重组部门分离。
Interspecific heterokaryons were produced between auxotrophic mutants of A. nidulans and A. rugulosus by polyethylene glycol induced fusion of somatic protoplasts. The heterokaryons grew very slowly producing colonies of irregular shape and, on complete medium, the parental strains were readily segregated. During long-term cultivation on minimal medium many of the heterokaryons gave rise to vigorously growing sectors characterized by the secretion of a brown pigment and by their stability when subcultured on complete medium. Protoplasts isolated from the heterokaryons gave rise to the same new colony type when regenerated on minimal medium, and to the new colony type plus the parental types on complete medium. The new colony type was assumed to be an interspecific hybrid and showed normal vegetative morphology, regular colony shape and size but produced new conidia. Conidia formed early in the development of the hybrid gave rise to hybrid colonies. Various sizes of conidia were observed; the largest were uninucleate and the smallest were enucleate. Comparisons of conidial size, numbers of nuclei in cells and DNA content per nucleus for hybrid and parental strains indicated that the hybrid was diploid. Conidia from older cultures of the hybrid were heterogeneous with hybrid, parental and recombinant colony types developing on germination. Cultivation of the hybrid in the presence of benomyl gave segregation of parental and recombinatant sectors.