Development and application of quantum dots for immunocytochemistry of human erythrocytes
Development and application of quantum dots for immunocytochemistry of human erythrocytes
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DOI：
10.1046/j.1365-2818.2003.01219.x
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发表时间：
2003-09-01
期刊：
影响因子：
--
通讯作者：
Dvorak, J
中科院分区：
文献类型：
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作者：
Tokumasu, F;Dvorak, J
量子点技术的最新发展导致了新的荧光免疫细胞化学探针的引入。与不具有光稳定性的有机荧光团相比，量子点的高量子产率和显著的光稳定性解决了与红细胞免疫细胞化学研究相关的主要问题。我们在这里报告了量子点在人类红细胞免疫细胞化学研究中的首次应用，能够用于高倍、三维红细胞重建技术。该过程包括用同功能的亚胺酯交联剂稳定人红细胞，以最大限度地减少固定物诱导的自身荧光，然后用量子点-单克隆抗体复合物反应以标记带3蛋白。我们的新方法清楚地显示了带3蛋白在红细胞膜上的非均匀的筏状分布。我们还证明了我们的技术在研究疟疾寄生虫入侵期间发生的红细胞膜修饰的适用性。
Recent developments in quantum dot technology have resulted in the introduction of new fluorescence immunocytochemical probes. In contrast to organic fluorophores, which are not photostable, the high quantum yield and remarkable photostability of quantum dots solve major problems associated with immunocytochemical studies of erythrocytes. We report here the first application of quantum dots to immunocytochemical studies of human erythrocytes capable of being used in high-magnification, three-dimensional erythrocyte reconstruction techniques. The procedure consists of stabilizing human erythrocytes with a homofunctional imidoester cross-linker to minimize fixative-induced autofluorescence followed by reacting with a quantum dot-monoclonal antibody complex to label band 3 protein. Our new procedure clearly showed a non-homogeneous, raft-like distribution of band 3 protein in the erythrocyte membrane. We also demonstrate the applicability of our technique to studies of erythrocyte membrane modifications occurring during the invasion of a malaria parasite.