Endothelium-dependent responses in long-term human coronary artery bypass grafts.
Endothelium-dependent responses in long-term human coronary artery bypass grafts.
复制标题
长期人冠状动脉旁路移植术中的内皮依赖性反应。
DOI：
10.1161/01.cir.83.2.402
复制
发表时间：
1991
期刊：
影响因子：
37.8
通讯作者：
Kirklin,JK
中科院分区：
文献类型：
--
作者：
Ku,DD;Caulfield,JB;Kirklin,JK
在本研究中，研究了长期人冠状动脉旁路移植术（cabg）对已知内皮依赖性血管扩张剂、乙酰胆碱、钙离子载体A23187、凝血酶和组胺，以及推定的内皮源性松弛因子一氧化氮的反应。16个cagb在1-2小时内从14例接受心脏移植的患者的心脏中分离出来。从这些cabg中共制备了109个环段并进行了体外研究。cabg的持续时间从7个月到12年不等。乙酰胆碱（0.01-10微米）、钙离子载体A23187（0.01-1.0微米）、凝血酶（0.01-1.0单位/毫升）和组胺（0.01-1.0微米）的加入持续产生剂量依赖性和内皮依赖性松弛，最大值分别达到-35.3 +/- 3.3%、-45.3 +/- 5.5%、-26.9 +/- 4.8%和-17.8 +/- 2.5%（平均+/- SEM）。不同移植时间的冠状动脉间无明显差异，但沿冠状动脉全长不同节段的舒张反应有明显差异。后一种内皮依赖性反应的差异似乎与这些冠状动脉绕道中内膜增生性病变的发展成负相关。添加一氧化氮（0.01-10微米）可产生有效的剂量和内皮无关的松弛作用，在伴有严重内膜增殖的cabg中也略有抑制。这些结果表明，长期移植的人隐静脉移植物保持其内皮依赖性反应，严重内膜增生性病变的发展，而不是移植物的持续时间，导致这些移植的cabg反应性的显著改变。
In the present study, responses of long-term human coronary artery bypass grafts (CABGs) to known endothelium-dependent vasodilators, acetylcholine, calcium ionophore A23187, thrombin, and histamine, as well as authentic nitric oxide, the putative endothelium-derived relaxing factor, were studied. Sixteen CAGBs were isolated within 1-2 hours from hearts of 14 patients receiving a cardiac transplant. A total of 109 ring segments were prepared from these CABGs and studied in vitro. The duration of the CABGs ranged from 7 months to 12 years. Addition of acetylcholine (0.01-10 microM), calcium ionophore A23187 (0.01-1.0 microM), thrombin (0.01-1.0 unit/ml), and histamine (0.01-1.0 microM) consistently produced a dose- and endothelium-dependent relaxation, reaching a maximum of -35.3 +/- 3.3%, -45.3 +/- 5.5%, -26.9 +/- 4.8%, and -17.8 +/- 2.5% (mean +/- SEM), respectively. No significant difference was observed among the CABGs with different duration of transplantation, whereas the relaxant responses of different segments along the entire length of a CABG were markedly different. These latter differences in the endothelium-dependent responses appear to correlate inversely with the development of intimal proliferative lesions in these CABGs. Addition of nitric oxide (0.01-10 microM) produced a potent dose- and endothelium-independent relaxation, which was also slightly depressed in CABGs with severe intimal proliferation. These results demonstrate that long-term transplanted human saphenous vein grafts retain their endothelium-dependent responses and that development of severe intimal proliferative lesions, rather than the duration of the grafts, result in marked alterations in the reactivity of these transplanted CABGs.